Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli
Producing recombinant proteins is a major accomplishment of biotechnology in the past
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …
century. Heterologous hosts, either eukaryotic or prokaryotic, are used for the production of …
An overview of culture conditions for recombinant protein expression in Escherichia coli
Escherichia coli is one of the most commonly used organisms for producing recombinant
protein. The recognized cell genome and well-known cell factory of the bacteria make it an …
protein. The recognized cell genome and well-known cell factory of the bacteria make it an …
Refolding of bioactive human epidermal growth factor from E. coli BL21 (DE3) inclusion bodies & evaluations on its in vitro & in vivo bioactivity
Human epidermal growth factor (hEGF) is a mitogenic protein widely used in pharmaceutical
and cosmetic industries, thus recombinant DNA technology has been applied to meet the …
and cosmetic industries, thus recombinant DNA technology has been applied to meet the …
Optimization of secreted recombinant human epidermal growth factor production using pectate lyase B from Escherichia coli BL21 (DE3) by central composite design …
Context: Human Epidermal Growth Factor (hEGF) is a potential therapeutic protein that has
been widely used as a healing agent for various chronic wounds. It induces the proliferation …
been widely used as a healing agent for various chronic wounds. It induces the proliferation …
Optimization of culture conditions for Mpt64 synthetic gene expression in Escherichia coli BL21 (DE3) using surface response methodology
MPT64 is a specific protein that is secreted by Mycobacterium tuberculosis complex (MTBC).
The objective of this study was to obtain optimum culture conditions for MPT64 synthetic …
The objective of this study was to obtain optimum culture conditions for MPT64 synthetic …
The effects of combined co-expression of groel/es and trigger factor chaperone towards Thermus thermophilus DNA polymerase enzyme expression on Escherichia …
IP Maksum, DF Utami, S Sriwidodo… - Journal of Applied …, 2024 - japsonline.com
The Thermus thermophilus (Tth) DNA polymerase enzyme, originating from the thermophilic
bacterium T. thermophilus, exhibits bifunctionally as both a DNA polymerase and a reverse …
bacterium T. thermophilus, exhibits bifunctionally as both a DNA polymerase and a reverse …
Safety aspect of recombinant protein produced by Escherichia coli: toxin evaluation with strain and genomic approach
Escherichia coli is a Gram-negative bacteria which is well known for its pathogenic
properties that can cause serious food poisoning, mostly indicated by diarrhea or other …
properties that can cause serious food poisoning, mostly indicated by diarrhea or other …
Continuous Secretion of Human Epidermal Growth Factor Based on Escherichia coli Biofilm
C Zhang, J Liao, Y Li, S Liu, M Li, D Zhang, Z Wang… - Fermentation, 2024 - mdpi.com
Human epidermal growth factor (hEGF) holds significant importance in the fields of medicine
and cosmetics. Therefore, it becomes imperative to develop a highly efficient fermentation …
and cosmetics. Therefore, it becomes imperative to develop a highly efficient fermentation …
Stabilization of eye drops containing autologous serum and recombinant human epidermal growth factor for dry eye syndrome
S Sriwidodo, ISK Syah, IP Maksum… - Journal of Advanced …, 2020 - journals.lww.com
Human epidermal growth factor (hEGF) and autologous serum are considered safer and
more effective in treating dry eye syndrome. However, suitable formulas and preparation …
more effective in treating dry eye syndrome. However, suitable formulas and preparation …
Activity and Effectiveness of Recombinant hEGF Excreted by Escherichia coli BL21 on Wound Healing in Induced Diabetic Mice
Context Human epidermal growth factor (hEGF) has biological activities and can be used in
medicines and cosmetics. A high level of effectiveness of hEGF can be obtained when three …
medicines and cosmetics. A high level of effectiveness of hEGF can be obtained when three …