Maintaining proteome integrity is essential for long-term viability of all organisms and is
overseen by intrinsic quality control mechanisms. The secretory pathway of eukaryotes …

Gene therapy has long held promise to correct a variety of human diseases and defects.
Discovery of the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR), the …

Zika virus (ZIKV), a previously obscure flavivirus closely related to dengue, West Nile,
Japanese encephalitis and yellow fever viruses, has emerged explosively since 2007 to …

Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art
instrumentation or the need for years of hands-on experience, for better or worse, ensures …

Flaviviruses, such as dengue, Japanese encephalitis, tick-borne encephalitis, West Nile,
yellow fever, and Zika viruses, are critically important human pathogens that sicken a …

Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Abstract Background Single-guide RNA (sgRNA) is one of the two key components of the
clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 genome-editing …

The Flaviviridae are a family of viruses that cause severe human diseases. For example,
dengue virus (DENV) is a rapidly emerging pathogen causing an estimated 100 million …

The frontiers of precision medicine have been revolutionized by the development of
Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR)/Cas9 as an editing …

The flaviviruses dengue virus (DENV) and Zika virus (ZIKV) are severe health threats with
rapidly expanding ranges. To identify the host cell dependencies of DENV and ZIKV, we …