Fluorinated analogues of the canonical α-L-amino acids have gained widespread attention
as building blocks that may endow peptides and proteins with advantageous biophysical …

With the spectacular advancement of NMR techniques and the flourishing of fluorine
chemistry allowing the synthesis of various fluorinated molecules, 19F NMR represents a …

With the development of NMR methodology and technology during the past decades, solid-
state NMR (ssNMR) has become a particularly important tool for investigating structure and …

Nuclear magnetic resonance (NMR) spectroscopy is a versatile tool for probing structure,
dynamics, folding, and interactions at atomic resolution. While naturally occurring …

While traditional 1H, 13C, and 15N (HCN) solution state NMR protocols provide a wealth of
data on protein structure and dynamics, 19F NMR provides a unique perspective of …

Fluorine is a valuable probe for investigating the interactions of biological molecules
because of its favorable NMR characteristics, its small size, and its near total absence from …

19F NMR is a powerful tool for monitoring protein conformational changes and interactions;
however, the inability to site-specifically introduce fluorine labels into proteins of biological …

Although fluorine plays only a minor role in biology, its physicochemical properties have
proven incredibly useful in the design of analogues of biologically important molecules …

A straightforward protocol for the site-specific incorporation of a 19F label into any protein in
vivo is described. This is done using a plasmid containing an orthogonal aminoacyl-tRNA …

Abstract PGLa and magainin 2 (MAG2) are amphiphilic α-helical membranolytic peptides
from frog skin with known synergistic antimicrobial activity. By systematically mutating …