Recent progress and challenges in CRISPR-Cas9 engineered algae and cyanobacteria
VK Patel, A Das, R Kumari, S Kajla - Algal Research, 2023 - Elsevier
Photosynthetic microorganisms like microalgae and cyanobacteria are quickly gaining
recognition for their financial potential and have recently been subjected to research into …
recognition for their financial potential and have recently been subjected to research into …
[HTML][HTML] Molecular advancements establishing Chlamydomonas as a host for biotechnological exploitation
Chlamydomonas reinhardtii is emerging as a production platform for biotechnological
purposes thanks to recent achievements, which we briefly summarize in this review. Firstly …
purposes thanks to recent achievements, which we briefly summarize in this review. Firstly …
Systems-wide analysis revealed shared and unique responses to moderate and acute high temperatures in the green alga Chlamydomonas reinhardtii
N Zhang, EM Mattoon, W McHargue, B Venn… - Communications …, 2022 - nature.com
Different intensities of high temperatures affect the growth of photosynthetic cells in nature.
To elucidate the underlying mechanisms, we cultivated the unicellular green alga …
To elucidate the underlying mechanisms, we cultivated the unicellular green alga …
Site-specific gene knock-in and bacterial phytase gene expression in Chlamydomonas reinhardtii via Cas9 RNP-mediated HDR
H Zadabbas Shahabadi, A Akbarzadeh… - Frontiers in Plant …, 2023 - frontiersin.org
In the present study, we applied the HDR (homology-directed DNA repair) CRISPR-Cas9-
mediated knock-in system to accurately insert an optimized foreign bacterial phytase gene at …
mediated knock-in system to accurately insert an optimized foreign bacterial phytase gene at …
Efficient methods for multiple types of precise gene‐editing in Chlamydomonas
H Chen, QL Yang, JX Xu, X Deng, YJ Zhang… - The Plant …, 2023 - Wiley Online Library
Precise gene‐editing using CRISPR/Cas9 technology remains a long‐standing challenge,
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …
Transgenic eukaryotic microalgae as green factories: providing new ideas for the production of biologically active substances
Q Shi, C Chen, W Zhang, P Wu, M Sun, H Wu… - Journal of Applied …, 2021 - Springer
Eukaryotic microalgae are important primary producers in nature that play an important role
in the energy cycle of nature. The eukaryotic microalgae are also regarded as potential …
in the energy cycle of nature. The eukaryotic microalgae are also regarded as potential …
Role of secondary metabolites in distressed microalgae
M Kolackova, A Janova, M Dobesova, M Zvalova… - Environmental …, 2023 - Elsevier
Proficient photosynthetic microalgae/cyanobacteria produce a remarkable amount of various
biomolecules. Secondary metabolites (SM) represent high value products for global biotrend …
biomolecules. Secondary metabolites (SM) represent high value products for global biotrend …
PCD Genes—From Patients to Model Organisms and Back to Humans
M Niziolek, M Bicka, A Osinka, Z Samsel… - International journal of …, 2022 - mdpi.com
Primary ciliary dyskinesia (PCD) is a hereditary genetic disorder caused by the lack of motile
cilia or the assembxly of dysfunctional ones. This rare human disease affects 1 out of 10,000 …
cilia or the assembxly of dysfunctional ones. This rare human disease affects 1 out of 10,000 …
Bio‐engineering of microalgae: Challenges and future prospects toward industrial and environmental applications
L Muthukrishnan - Journal of Basic Microbiology, 2022 - Wiley Online Library
Microalgae are complex metabolic machineries whose potential has been continuously
tapped for wide range of applications. Their suboptimal biomass yield and bioactives …
tapped for wide range of applications. Their suboptimal biomass yield and bioactives …
The Spermidine Synthase Gene SPD1: A Novel Auxotrophic Marker for Chlamydomonas reinhardtii Designed by Enhanced CRISPR/Cas9 Gene Editing
Biotechnological application of the green microalga Chlamydomonas reinhardtii hinges on
the availability of selectable markers for effective expression of multiple transgenes …
the availability of selectable markers for effective expression of multiple transgenes …