C4-Dicarboxylates as Growth Substrates and Signaling Molecules for Commensal and Pathogenic Enteric Bacteria in Mammalian Intestine

C Schubert, G Unden - Journal of bacteriology, 2022 - Am Soc Microbiol
ABSTRACT The C4-dicarboxylates (C4-DC) l-aspartate and l-malate have been identified
as playing an important role in the colonization of mammalian intestine by enteric bacteria …

Protein activity sensing in bacteria in regulating metabolism and motility

A Alvarado, W Behrens, C Josenhans - Frontiers in microbiology, 2020 - frontiersin.org
Bacteria have evolved complex sensing and signaling systems to react to their changing
environments, most of which are present in all domains of life. Canonical bacterial sensing …

Chromosomal integrons are genetically and functionally isolated units of genomes

P Blanco, F Trigo da Roza… - Nucleic acids …, 2024 - academic.oup.com
Integrons are genetic elements that increase the evolvability of bacteria by capturing new
genes and stockpiling them in arrays. Sedentary chromosomal integrons (SCIs) can be …

L‐Aspartate as a high‐quality nitrogen source in Escherichia coli: Regulation of L‐aspartase by the nitrogen regulatory system and interaction of L‐aspartase with …

C Schubert, S Zedler, A Strecker… - Molecular …, 2021 - Wiley Online Library
Escherichia coli uses the C4‐dicarboxylate transporter DcuA for L‐aspartate/fumarate
antiport, which results in the exploitation of L‐aspartate for fumarate respiration under …

C4‐dicarboxylates and l‐aspartate utilization by Escherichia coli K‐12 in the mouse intestine: l‐aspartate as a major substrate for fumarate respiration and as a …

C Schubert, M Winter, A Ebert‐Jung… - Environmental …, 2021 - Wiley Online Library
C4‐dicarboxylates, such as fumarate, l‐malate and l‐aspartate represent substrates for
anaerobic growth of Escherichia coli by fumarate respiration. Here, we determined whether …

Metabolic engineering of Escherichia coli for efficient production of l-arginine

HD Wang, JZ Xu, WG Zhang - Applied Microbiology and Biotechnology, 2022 - Springer
As an important semi-essential amino acid, l-arginine (l-Arg) has important application
prospects in medicine and health care. However, it remains a challenge to efficiently …

A signaling complex of adenylate cyclase CyaC of Sinorhizobium meliloti with cAMP and the transcriptional regulators Clr and CycR

R Klein, J Brehm, J Wissig, R Heermann, G Unden - BMC microbiology, 2023 - Springer
Abstract Background Adenylate cyclases (ACs) generate the second messenger cyclic AMP
(cAMP), which is found in all domains of life and is involved in the regulation of various cell …

Escherichia coli uses a dedicated importer and desulfidase to ferment cysteine

Y Zhou, JA Imlay - MBio, 2022 - Am Soc Microbiol
CyuA of Escherichia coli is an inducible desulfidase that degrades cysteine to pyruvate,
ammonium, and hydrogen sulfide. Workers have conjectured that its role may be to defend …

Deep learning for rapid identification of microbes using metabolomics profiles

D Wang, P Greenwood, MS Klein - Metabolites, 2021 - mdpi.com
Rapid detection of viable microbes remains a challenge in fields such as microbial food
safety. We here present the application of deep learning algorithms to the rapid detection of …

Metabolically Modifying the Central and Competitive Metabolic Pathways for Enhanced D-Pantoic Acid Synthesis

HY Zhou, YH Chen, DD Chen, ZW Wang… - Journal of Agricultural …, 2025 - ACS Publications
D-Pantoic acid is an essential precursor for the synthesis of vitamin B5. However, the
microbial synthesis of D-pantoic acid suffers from a low yield. Herein, to improve D-pantoic …