The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc
building blocks are available at low cost because of the economies of scale arising from …

Methods to prepare proteins that include a specific modification at a desired position are
essential for understanding their cellular functions and physical properties in living systems …

This protocol provides a detailed procedure for the chemical synthesis of proteins through
native chemical ligation of peptide hydrazides. The two crucial stages of this protocol are (i) …

1. INTRODUCTION In eukaryotic cells, inheritable information is stored in a nucleoprotein
complex referred to as chromatin. 1 This genome architecture serves two key purposes. On …

Conspectus Chemical manipulation of naturally occurring peptides offers a convenient route
for generating analogs to screen against different therapeutic targets. However, the limited …

The field of site‐specific modification of proteins has drawn significant attention in recent
years owing to its importance in various research areas such as the development of novel …

Successive peptide ligation using a one‐pot method can improve the efficiency of protein
chemical synthesis. Although one‐pot three‐segment ligation has enjoyed widespread …

The microtubule-associated protein Tau plays a central role in neurodegeneration and is a
leading therapeutic target for the treatment of Alzheimer's disease (AD). Several lines of …

Fully unprotected peptide o‐aminoanilides can be efficiently activated by NaNO2 in
aqueous solution to furnish peptide thioesters for use in native chemical ligation. This finding …