Escherichia coli as a host for metabolic engineering
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
Microbial polyhydroxyalkanoates and nonnatural polyesters
Microorganisms produce diverse polymers for various purposes such as storing genetic
information, energy, and reducing power, and serving as structural materials and scaffolds …
information, energy, and reducing power, and serving as structural materials and scaffolds …
Microbial acetyl-CoA metabolism and metabolic engineering
Recent concerns over the sustainability of petrochemical-based processes for production of
desired chemicals have fueled research into alternative modes of production. Metabolic …
desired chemicals have fueled research into alternative modes of production. Metabolic …
Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria
Carbon-negative synthesis of biochemical products has the potential to mitigate global CO2
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …
emissions. An attractive route to do this is the reverse β-oxidation (r-BOX) pathway coupled …
An orthogonal metabolic framework for one-carbon utilization
Metabolic engineering often entails concurrent engineering of substrate utilization, central
metabolism and product synthesis pathways, inevitably creating interdependency with …
metabolism and product synthesis pathways, inevitably creating interdependency with …
Cell-free gene expression: methods and applications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …
molecules and processes outside of living intact cells. The foundational principle is that …
Energy-and carbon-efficient synthesis of functionalized small molecules in bacteria using non-decarboxylative Claisen condensation reactions
Anabolic metabolism can produce an array of small molecules, but yields and productivities
are low owing to carbon and energy inefficiencies and slow kinetics,. Catabolic and …
are low owing to carbon and energy inefficiencies and slow kinetics,. Catabolic and …
Integrated engineering of β-oxidation reversal and ω-oxidation pathways for the synthesis of medium chain ω-functionalized carboxylic acids
JM Clomburg, MD Blankschien, JE Vick, A Chou… - Metabolic …, 2015 - Elsevier
An engineered reversal of the β-oxidation cycle was exploited to demonstrate its utility for
the synthesis of medium chain (6–10-carbons) ω-hydroxyacids and dicarboxylic acids from …
the synthesis of medium chain (6–10-carbons) ω-hydroxyacids and dicarboxylic acids from …
Next generation biofuel engineering in prokaryotes
LS Gronenberg, RJ Marcheschi, JC Liao - Current opinion in chemical …, 2013 - Elsevier
Next-generation biofuels must be compatible with current transportation infrastructure and
be derived from environmentally sustainable resources that do not compete with food crops …
be derived from environmentally sustainable resources that do not compete with food crops …
Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels
Increasing global energy demand and environmental concerns associated with petroleum
have raised interest in biofuels for reducing dependency on crude oil and promote carbon …
have raised interest in biofuels for reducing dependency on crude oil and promote carbon …