With reference to reference genes: a systematic review of endogenous controls in gene expression studies
JR Chapman, J Waldenström - PloS one, 2015 - journals.plos.org
The choice of reference genes that are stably expressed amongst treatment groups is a
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …
crucial step in real-time quantitative PCR gene expression studies. Recent guidelines have …
Single-cell RNA sequencing analysis reveals a crucial role for CTHRC1 (collagen triple helix repeat containing 1) cardiac fibroblasts after myocardial infarction
Background: Cardiac fibroblasts (CFs) have a central role in the ventricular remodeling
process associated with different types of fibrosis. Recent studies have shown that …
process associated with different types of fibrosis. Recent studies have shown that …
Basic biology of GAPDH
NW Seidler, NW Seidler - GAPDH: Biological Properties and Diversity, 2013 - Springer
The GAPDH gene is highly conserved with a promoter that contains several types of
regulatory elements, perhaps even in a distal intron. Curiously, the transcription start site …
regulatory elements, perhaps even in a distal intron. Curiously, the transcription start site …
Characterization of housekeeping genes in zebrafish: male-female differences and effects of tissue type, developmental stage and chemical treatment
AT McCurley, GV Callard - BMC molecular biology, 2008 - Springer
Background Research using the zebrafish model has experienced a rapid growth in recent
years. Although real-time reverse transcription PCR (QPCR), normalized to an internal …
years. Although real-time reverse transcription PCR (QPCR), normalized to an internal …
Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment
L Qin, J He, RN Hanes, O Pluzarev, JS Hong… - Journal of …, 2008 - Springer
Background Cytokines and alcohol share a common modulation of inflammation and
hormones as well as being implicated in multiple diseases, but the mechanisms are poorly …
hormones as well as being implicated in multiple diseases, but the mechanisms are poorly …
Cytidine-containing tails robustly enhance and prolong protein production of synthetic mRNA in cell and in vivo
Synthetic mRNAs are rising rapidly as alternative therapeutic agents for delivery of proteins.
However, the practical use of synthetic mRNAs has been restricted by their low cellular …
However, the practical use of synthetic mRNAs has been restricted by their low cellular …
Profiling of RNA Degradation for Estimation of Post Morterm Interval
F Sampaio-Silva, T Magalhaes, F Carvalho… - PloS one, 2013 - journals.plos.org
An estimation of the post mortem interval (PMI) is frequently touted as the Holy Grail of
forensic pathology. During the first hours after death, PMI estimation is dependent on the …
forensic pathology. During the first hours after death, PMI estimation is dependent on the …
Robust RT-qPCR data normalization: validation and selection of internal reference genes during post-experimental data analysis
D Ling, PM Salvaterra - PloS one, 2011 - journals.plos.org
Reverse transcription and real-time PCR (RT-qPCR) has been widely used for rapid
quantification of relative gene expression. To offset technical confounding variations, stably …
quantification of relative gene expression. To offset technical confounding variations, stably …
Evaluation of endogenous references for gene expression profiling in different tissues of the oriental fruit fly Bactrocera dorsalis (Diptera: Tephritidae)
GM Shen, HB Jiang, XN Wang, JJ Wang - BMC molecular biology, 2010 - Springer
Background Quantitative real-time reverse transcriptase PCR (RT-qPCR) has been widely
used for quantification of mRNA as a way to determine key genes involved in different …
used for quantification of mRNA as a way to determine key genes involved in different …
The toll-like receptor family protein RP105/MD1 complex is involved in the immunoregulatory effect of exopolysaccharides from Lactobacillus plantarum N14
Abstract The radioprotective 105 (RP105)/MD1 complex is a member of the Toll-like receptor
(TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) …
(TLR) family. It was reported that RP105/MD1 cooperates with the lipopolysaccharide (LPS) …