The landmark 1969 discovery of nuclear RNA polymerases I, II and III in diverse eukaryotes
represented a major turning point in the field that, with subsequent elucidation of the distinct …

In eukaryotes, transcription of the diverse array of tens of thousands of protein-coding genes
is carried out by RNA polymerase II. The control of this process is predominantly mediated …

We have developed a procedure for preparing extracts from nuclei of human tissue culture
cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of …

We have studied the transient expression of a cloned rabbit hemoglobin/31 gene after its
introduction into HeLa cells. Two and one-half days after transfection using the calcium …

A point mutation in the simian virus 40 large-T gene, which was generated by mixed
oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a …

Fractionation of HeLa cell extracts reveals the presence of a promoter-specific transcription
factor, Spi, which activates a class of promoters that includes the SV40 early promoter but …

The complete DNA sequence of the human JC virus, which was found to consist of 5,130
nucleotide pairs, is presented. The amino acid sequence of six proteins could be deduced …

A new fractionation procedure separates a wholecell HeLa extract into three components
required for accurate in vitro transcription. One component (Spl) is a promoter-specific factor …

In a lysogen, most genes of phage λ are repressed; in response to a transient induction
signal, they are efficiently switched on. The switch, which consists in part of a tripartite …

We have purified a 52 kd protein, AP-2, that binds to enhancer regions of SV40 and human
metallothionein IIA (hMT IIA) and stimulates RNA synthesis from these promoters in vitro …