We have implemented scanning fluorescence correlation spectroscopy (sFCS) for precise
determination of diffusion coefficients of fluorescent molecules in solution. The measurement …

Semiconductor nanocrystals (quantum dots) have been increasingly employed in measuring
the dynamic behavior of biomacromolecules using fluorescence correlation spectroscopy …

Two-photon probe excitation data are commonly presented as absorption cross section or
molecular brightness (the detected fluorescence rate per molecule). We report two-photon …

One of the key questions regarding intracellular diffusion is how the environment affects
molecular mobility. Mostly, intracellular diffusion has been described as hindered, and the …

Kazimierz Wielki University of Bydgoszcz, Bydgoszcz, Poland. Tropomyosins (TMs) are a
family of proteins which regulate actin interactions with myosins and other actin-binding …

Calmodulin (CaM) is the major pathway that transduces intracellular Ca 2+ increases to the
activation of a wide variety of downstream signaling enzymes. CaM and its target proteins …

A protocol on how to perform high-precision interdye distance measurements using Förster
resonance energy transfer (FRET) at the single-molecule level in multiparameter …

Advancement of RNAi therapies is mainly hindered by the development of efficient delivery
vehicles. The ability to create small size (< 30 nm) oligonucleotide nanoparticles is essential …

Two‐photon excitation in fluorescence correlation spectroscopy (FCS) is often preferred to
one‐photon excitation because of reduced bulk photobleaching and photodamage, and …

Demands in flow cytometry for increased multiplexing (for detection of multiple antigens) and
brightness (for detection of rare entities) require new fluorophores (ie,“colors”) with spectrally …