Mass spectrometry-based proteomics
R Aebersold, M Mann - Nature, 2003 - nature.com
Recent successes illustrate the role of mass spectrometry-based proteomics as an
indispensable tool for molecular and cellular biology and for the emerging field of systems …
indispensable tool for molecular and cellular biology and for the emerging field of systems …
Proteomic analysis of post-translational modifications
Post-translational modifications modulate the activity of most eukaryote proteins. Analysis of
these modifications presents formidable challenges but their determination generates …
these modifications presents formidable challenges but their determination generates …
A statistical model for identifying proteins by tandem mass spectrometry
AI Nesvizhskii, A Keller, E Kolker… - Analytical …, 2003 - ACS Publications
A statistical model is presented for computing probabilities that proteins are present in a
sample on the basis of peptides assigned to tandem mass (MS/MS) spectra acquired from a …
sample on the basis of peptides assigned to tandem mass (MS/MS) spectra acquired from a …
Proteomics to study genes and genomes
Proteomics, the large-scale analysis of proteins, will contribute greatly to our understanding
of gene function in the post-genomic era. Proteomics can be divided into three main …
of gene function in the post-genomic era. Proteomics can be divided into three main …
Molecular biologist's guide to proteomics
PR Graves, TAJ Haystead - Microbiology and molecular biology …, 2002 - Am Soc Microbiol
The emergence of proteomics, the large-scale analysis of proteins, has been inspired by the
realization that the final product of a gene is inherently more complex and closer to function …
realization that the final product of a gene is inherently more complex and closer to function …
Analysis of proteins and proteomes by mass spectrometry
M Mann, RC Hendrickson… - Annual review of …, 2001 - annualreviews.org
▪ Abstract A decade after the discovery of electrospray and matrix-assisted laser desorption
ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules …
ionization (MALDI), methods that finally allowed gentle ionization of large biomolecules …
Immunoaffinity profiling of tyrosine phosphorylation in cancer cells
J Rush, A Moritz, KA Lee, A Guo, VL Goss… - Nature …, 2005 - nature.com
Tyrosine kinases play a prominent role in human cancer, yet the oncogenic signaling
pathways driving cell proliferation and survival have been difficult to identify, in part because …
pathways driving cell proliferation and survival have been difficult to identify, in part because …
Analysis of protein phosphorylation using mass spectrometry: deciphering the phosphoproteome
In signal transduction in eukaryotes, protein phosphorylation is a key event. To understand
signaling processes, we must first acquire an inventory of phosphoproteins and their …
signaling processes, we must first acquire an inventory of phosphoproteins and their …
Modification‐specific proteomics: strategies for characterization of post‐translational modifications using enrichment techniques
More than 300 different types of protein post‐translational modifications (PTMs) have been
described, many of which are known to have pivotal roles in cellular physiology and …
described, many of which are known to have pivotal roles in cellular physiology and …
Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns
MWH Pinkse, PM Uitto, MJ Hilhorst, B Ooms… - Analytical …, 2004 - ACS Publications
Selective detection of phosphopeptides from proteolytic digests is a challenging and highly
relevant task in many proteomics applications. Often phosphopeptides are present in small …
relevant task in many proteomics applications. Often phosphopeptides are present in small …