Micro total analysis systems. 2. Analytical standard operations and applications

PA Auroux, D Iossifidis, DR Reyes, A Manz - Analytical chemistry, 2002 - ACS Publications
After having reviewed some aspects of microfluidic system preparation in the first part (1), in
this second part of the review we will cover a number of standard operations (namely …

Beyond gel electrophoresis: Microfluidic separations, fluorescence burst analysis, and DNA stretching

KD Dorfman, SB King, DW Olson, JDP Thomas… - Chemical …, 2013 - ACS Publications
This Review addresses methods for obtaining sequence information directly from
unamplified genomic length DNA. Our generic starting point is a large piece of DNA that …

Integrated system for rapid PCR-based DNA analysis in microfluidic devices

J Khandurina, TE McKnight, SC Jacobson… - Analytical …, 2000 - ACS Publications
An integrated system for rapid PCR-based analysis on a microchip has been demonstrated.
The system couples a compact thermal cycling assembly based on dual Peltier …

Counting low-copy number proteins in a single cell

B Huang, H Wu, D Bhaya, A Grossman, S Granier… - Science, 2007 - science.org
We have designed a microfluidic device in which we can manipulate, lyse, label, separate,
and quantify the protein contents of a single cell using single-molecule fluorescence …

Monolithic porous polymer for on-chip solid-phase extraction and preconcentration prepared by photoinitiated in situ polymerization within a microfluidic device

C Yu, MH Davey, F Svec, JMJ Frechet - Analytical Chemistry, 2001 - ACS Publications
Monolithic porous polymers have been prepared by photoinitiated polymerization within the
channels of a microfluidic device and used for on-chip solid-phase extraction and …

Bioanalysis in microfluidic devices

J Khandurina, A Guttman - Journal of Chromatography A, 2002 - Elsevier
Microfabricated bioanalytical devices (also referred to as laboratory-on-a-chip or μ-TAS)
offer highly efficient platforms for simultaneous analysis of a large number of biologically …

Miniaturized continuous flow reaction vessels: influence on chemical reactions

M Brivio, W Verboom, DN Reinhoudt - Lab on a Chip, 2006 - pubs.rsc.org
This review offers an overview of the relatively young research area of continuous flow lab-
on-a-chip for synthetic applications. A short introduction on the basic aspects of lab-on-a …

Recent developments in electrokinetically driven analysis on microfabricated devices

GJM Bruin - ELECTROPHORESIS: An International Journal, 2000 - Wiley Online Library
This review is devoted to the rapid developments in the field of microfluidic separation
devices in which the flow is electrokinetically driven, and where the separation element …

[图书][B] Bio-MEMS: technologies and applications

W Wang, SA Soper - 2006 - taylorfrancis.com
Microelectromechanical systems (MEMS) are evolving into highly integrated technologies
for a variety of application areas. Add the biological dimension to the mix and a host of new …

Microscale flow visualization

D Sinton - Microfluidics and Nanofluidics, 2004 - Springer
Advances in microfluidic and nanofluidic technologies have been paralleled by advances in
methods for direct optical measurement of transport phenomena on these scales. A variety …