Genotypic and phenotypic characteristics associated with biofilm formation by human clinical Escherichia coli isolates of different pathotypes

J Schiebel, A Böhm, J Nitschke… - Applied and …, 2017 - Am Soc Microbiol
Bacterial biofilm formation is a widespread phenomenon and a complex process requiring a
set of genes facilitating the initial adhesion, maturation, and production of the extracellular …

qPCRtools: An R package for qPCR data processing and visualization

X Li, Y Wang, J Li, X Mei, Y Liu, H Huang - Frontiers in Genetics, 2022 - frontiersin.org
In biological research, qPCR is a technique that is frequently used to measure gene
expression levels. The calculation of gene amplification efficiency is a critical step in the …

Combinations of PCR and isothermal amplification techniques are suitable for fast and sensitive detection of SARS-CoV-2 viral RNA

DA Varlamov, KA Blagodatskikh… - … in bioengineering and …, 2020 - frontiersin.org
The newly identified coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-
CoV-2), causes coronavirus disease 2019 (COVID-19) and has affected over 25 million …

A round‐robin evaluation of the repeatability and reproducibility of environmental DNA assays for dreissenid mussels

AJ Sepulveda, PR Hutchins, C Jackson… - Environmental …, 2020 - Wiley Online Library
Resource managers may be hesitant to make decisions based on environmental (e) DNA
results alone since eDNA is an indirect method of species detection. One way to reduce the …

A semi-automated, isolation-free, high-throughput SARS-CoV-2 reverse transcriptase (RT) loop-mediated isothermal amplification (LAMP) test

J Schmidt, S Berghaus, F Blessing, F Wenzel… - Scientific Reports, 2021 - nature.com
Shortages of reverse transcriptase (RT)-polymerase chain reaction (PCR) reagents and
related equipment during the COVID-19 pandemic have demonstrated the need for …

Risk factors for antimicrobial resistance in turkey farms: a cross-sectional study in three European countries

M Horie, D Yang, P Joosten, P Munk, K Wadepohl… - Antibiotics, 2021 - mdpi.com
Food-producing animals are an important reservoir and potential source of transmission of
antimicrobial resistance (AMR) to humans. However, research on AMR in turkey farms is …

Light regulation of rhodopsin distribution during outer segment renewal in murine rod photoreceptors

K Rose, N Chen, A Andreev, J Chen, VJ Kefalov… - Current Biology, 2024 - cell.com
Vision under dim light relies on primary cilia elaborated by rod photoreceptors in the retina.
This specialized sensory structure, called the rod outer segment (ROS), comprises hundreds …

[PDF][PDF] R as an environment for reproducible analysis of DNA amplification experiments.

S Rödiger, M Burdukiewicz, K Blagodatskikh, M Jahn… - R J., 2015 - journal.r-project.org
There is an ever-increasing number of applications, which use quantitative PCR (qPCR) or
digital PCR (dPCR) to elicit fundamentals of biological processes. Moreover, quantitative …

System-specific periodicity in quantitative real-time polymerase chain reaction data questions threshold-based quantitation

AN Spiess, S Rödiger, M Burdukiewicz, T Volksdorf… - Scientific Reports, 2016 - nature.com
Real-time quantitative polymerase chain reaction (qPCR) data are found to display periodic
patterns in the fluorescence intensity as a function of sample number for fixed cycle number …

High-throughput quantification of bacterial-cell interactions using virtual colony counts

S Hoffmann, S Walter, AK Blume, S Fuchs… - Frontiers in cellular …, 2018 - frontiersin.org
The quantification of bacteria in cell culture infection models is of paramount importance for
the characterization of host-pathogen interactions and pathogenicity factors involved. The …