Integrative structure determination is a powerful approach to modeling the structures of
biological systems based on data produced by multiple experimental and theoretical …

Mass spectrometric epitope mapping has become a versatile method to precisely determine
a soluble antigen's partial structure that directly interacts with an antibody in solution. Typical …

Coevolutionary sequence analysis has become a commonly used technique for de novo
prediction of the structure and function of proteins, RNA, and protein complexes. We present …

We describe an experimental method of three-dimensional (3D) structure determination that
exploits the increasing ease of high-throughput mutational scans. Inspired by the success of …

Residue pairs that directly coevolve in protein families are generally close in protein 3D
structures. Here we study the exceptions to this general trend—directly coevolving residue …

Atomic-resolution structure determination is crucial for understanding protein function. Cryo-
EM and NMR spectroscopy both provide structural information, but currently cryo-EM does …

Aberrant activation of Ras GTPase is closely associated with human cancers. Mechanistic
and structural insights into conformational transitions underlying the Ras deactivation …

Methyl-specific isotope labeling is a powerful tool to study the structure, dynamics and
interactions of large proteins and protein complexes by solution-state NMR. However …

Potts Hamiltonian models of protein sequence co-variation are statistical models constructed
from the pair correlations observed in a multiple sequence alignment (MSA) of a protein …

Uncovering molecular basis with regard to the conformational change of two switches I and
II in the GppNHp (GNP)-bound H-Ras is highly significant for the understanding of Ras …