Molecular mechanisms of substrate-controlled ring dynamics and substepping in a nucleic acid-dependent hexameric motor
ND Thomsen, MR Lawson… - Proceedings of the …, 2016 - National Acad Sciences
Ring-shaped hexameric helicases and translocases support essential DNA-, RNA-, and
protein-dependent transactions in all cells and many viruses. How such systems coordinate …
protein-dependent transactions in all cells and many viruses. How such systems coordinate …
In vitro reconstitution and crystallization of Cas9 endonuclease bound to a guide RNA and a DNA target
C Anders, O Niewoehner, M Jinek - Methods in enzymology, 2015 - Elsevier
The programmable RNA-guided DNA cleavage activity of the bacterial CRISPR-associated
endonuclease Cas9 is the basis of genome editing applications in numerous model …
endonuclease Cas9 is the basis of genome editing applications in numerous model …
Sequence-dependent nanometer-scale conformational dynamics of individual RecBCD–DNA complexes
AR Carter, MH Seaberg, HF Fan, G Sun… - Nucleic acids …, 2016 - academic.oup.com
RecBCD is a multifunctional enzyme that possesses both helicase and nuclease activities.
To gain insight into the mechanism of its helicase function, RecBCD unwinding at low …
To gain insight into the mechanism of its helicase function, RecBCD unwinding at low …
Thermal shift assay for characterizing the stability of RNA helicases and their interaction with ligands
E Saridakis, F Coste - RNA Remodeling Proteins: Methods and Protocols, 2021 - Springer
Thermofluor or thermal shift assay is an easily implementable, high-throughput method for
assessing the thermostability of proteins and the influence of various ligands on that stability …
assessing the thermostability of proteins and the influence of various ligands on that stability …
Knowledge-Based Models: RNA
R Méndez, A Krokhotin, M Convertino… - … -Grained Modeling of …, 2017 - taylorfrancis.com
This chapter reviews some of the most employed methodologies to model the three-
dimensional (3D) structure of ribonucleic acid (RNA) molecules, in particular those based on …
dimensional (3D) structure of ribonucleic acid (RNA) molecules, in particular those based on …
Structural Studies of RNases H2 as an Example of Crystal Structure Determination of Protein–Nucleic Acid Complexes
M Figiel, M Nowotny - Methods in Enzymology, 2017 - Elsevier
RNases H2 are nucleases that cleave nucleic acids that comprise both RNA and DNA. They
specifically recognize and cleave junctions between RNA and DNA using an intricate …
specifically recognize and cleave junctions between RNA and DNA using an intricate …
Interplay Between the Helicase-Primase and the DNA Polymerase-Processivity Factor During Herpes DNA Replication
SM Dickerson - 2017 - search.proquest.com
There are seven proteins essential for Herpes Simplex Virus Type 1 (HSV-1) DNA
replication. While some of their roles are known, many of their mechanisms remain unclear …
replication. While some of their roles are known, many of their mechanisms remain unclear …