Using genetically incorporated unnatural amino acids to control protein functions in mammalian cells

AR Nödling, LA Spear, TL Williams… - Essays in …, 2019 - portlandpress.com
Genetic code expansion allows unnatural (non-canonical) amino acid incorporation into
proteins of interest by repurposing the cellular translation machinery. The development of …

Using genetic code expansion for protein biochemical studies

CZ Chung, K Amikura, D Söll - Frontiers in Bioengineering and …, 2020 - frontiersin.org
Protein identification has gone beyond simply using protein/peptide tags and labeling
canonical amino acids. Genetic code expansion has allowed residue-or site-specific …

Fluorogenic photoaffinity labeling of proteins in living cells

TM Ayele, SD Knutson, S Ellipilli, H Hwang… - Bioconjugate …, 2019 - ACS Publications
Genetically encoded fluorescent proteins or small-molecule probes that recognize specific
protein binding partners can be used to label proteins to study their localization and function …

Site‐Specific Protein Labeling with Fluorophores as a Tool To Monitor Protein Turnover

YG Mideksa, M Fottner, S Braus, CAM Weiß… - …, 2020 - Wiley Online Library
Proteins that terminally fail to acquire their native structure are detected and degraded by
cellular quality control systems. Insights into cellular protein quality control are key to a better …