Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria
I Ahmed, J Hahn, A Henrickson, FT Khaja… - Nature …, 2022 - nature.com
An essential step in bacterial transformation is the uptake of DNA into the periplasm, across
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …
Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
A Bepperling, G Richter - European Biophysics Journal, 2023 - Springer
Lipid nanoparticles as delivery system for mRNA have recently attracted attention to a
broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to …
broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to …
Analytical ultracentrifugation in colloid and polymer science: new possibilities and perspectives after 100 years
H Cölfen - Colloid and Polymer Science, 2023 - Springer
Analytical ultracentrifugation (AUC) is a classical polymer and colloid analysis technique
invented by Theodor Svedberg 100 years ago. Modern hard-and software and powerful …
invented by Theodor Svedberg 100 years ago. Modern hard-and software and powerful …
Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan
Aim: We present multi-wavelength (MW) analytical ultracentrifugation (AUC) methods
offering superior accuracy for adeno-associated virus characterization and quantification …
offering superior accuracy for adeno-associated virus characterization and quantification …
A new UltraScan module for the characterization and quantification of analytical buoyant density equilibrium experiments to determine AAV capsid loading
A Savelyev, EH Brookes, A Henrickson… - European Biophysics …, 2023 - Springer
A method for characterizing and quantifying peaks formed in an analytical buoyant density
equilibrium (ABDE) experiment is presented. An algorithm is derived to calculate the …
equilibrium (ABDE) experiment is presented. An algorithm is derived to calculate the …
Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs
A Bepperling, J Best - European Biophysics Journal, 2023 - Springer
Due to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors,
boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been …
boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been …
Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments
B Demeler - Current Protocols, 2024 - Wiley Online Library
Analytical ultracentrifugation experiments play an integral role in the solution‐phase
characterization of biological macromolecules and their interactions. This unit discusses the …
characterization of biological macromolecules and their interactions. This unit discusses the …
Investigation of dynamic solution interactions between NET-1 and UNC-5B by multi-wavelength analytical ultracentrifugation
H Gabir, M Gupta, M Meier, F Heide, M Koch… - European Biophysics …, 2023 - Springer
NET-1 is a key chemotropic ligand that signals commissural axon migration and change in
direction. NET-1 and its receptor UNC-5B switch axon growth cones from attraction to …
direction. NET-1 and its receptor UNC-5B switch axon growth cones from attraction to …
A spectral decomposition quality assessment tool for multi-wavelength AUC experiments with UltraScan
S Mortezazadeh, B Demeler - European Biophysics Journal, 2023 - Springer
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recently developed technique
that has proven to be a promising tool to investigate mixtures of molecules containing …
that has proven to be a promising tool to investigate mixtures of molecules containing …
Analytical Ultracentrifugation to Assess the Quality of LNP-mRNA Therapeutics
G Guerrini, D Mehn, D Scaccabarozzi, S Gioria… - International Journal of …, 2024 - mdpi.com
The approval of safe and effective LNP-mRNA vaccines during the SARS-CoV-2 pandemic
is catalyzing the development of the next generation of mRNA therapeutics. Proper …
is catalyzing the development of the next generation of mRNA therapeutics. Proper …