An essential step in bacterial transformation is the uptake of DNA into the periplasm, across
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …

Lipid nanoparticles as delivery system for mRNA have recently attracted attention to a
broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to …

Analytical ultracentrifugation (AUC) is a classical polymer and colloid analysis technique
invented by Theodor Svedberg 100 years ago. Modern hard-and software and powerful …

Aim: We present multi-wavelength (MW) analytical ultracentrifugation (AUC) methods
offering superior accuracy for adeno-associated virus characterization and quantification …

A method for characterizing and quantifying peaks formed in an analytical buoyant density
equilibrium (ABDE) experiment is presented. An algorithm is derived to calculate the …

Due to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors,
boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been …

Analytical ultracentrifugation experiments play an integral role in the solution‐phase
characterization of biological macromolecules and their interactions. This unit discusses the …

NET-1 is a key chemotropic ligand that signals commissural axon migration and change in
direction. NET-1 and its receptor UNC-5B switch axon growth cones from attraction to …

Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recently developed technique
that has proven to be a promising tool to investigate mixtures of molecules containing …

The approval of safe and effective LNP-mRNA vaccines during the SARS-CoV-2 pandemic
is catalyzing the development of the next generation of mRNA therapeutics. Proper …