Actin bundles dynamics and architecture

S Rajan, DS Kudryashov, E Reisler - Biomolecules, 2023 - mdpi.com
Cells use the actin cytoskeleton for many of their functions, including their division,
adhesion, mechanosensing, endo-and phagocytosis, migration, and invasion. Actin bundles …

Exploring giant unilamellar vesicle production for artificial cells—Current challenges and future directions

L Van de Cauter, L van Buren, GH Koenderink… - Small …, 2023 - Wiley Online Library
Creating an artificial cell from the bottom up is a long‐standing challenge and, while
significant progress has been made, the full realization of this goal remains elusive …

Minimal out-of-equilibrium metabolism for synthetic cells: a membrane perspective

E Bailoni, M Partipilo, J Coenradij… - ACS Synthetic …, 2023 - ACS Publications
Life-like systems need to maintain a basal metabolism, which includes importing a variety of
building blocks required for macromolecule synthesis, exporting dead-end products, and …

Encapsulated actomyosin patterns drive cell-like membrane shape changes

Y Bashirzadeh, H Moghimianavval, AP Liu - Iscience, 2022 - cell.com
Cell shape changes from locomotion to cytokinesis are, to a large extent, driven by myosin-
driven remodeling of cortical actin patterns. Passive crosslinkers such as α-actinin and …

Liquid-like condensates mediate competition between actin branching and bundling

K Graham, A Chandrasekaran… - Proceedings of the …, 2024 - National Acad Sciences
Cellular remodeling of actin networks underlies cell motility during key morphological
events, from embryogenesis to metastasis. In these transformations, there is an inherent …

Methods to mechanically perturb and characterize GUV-based minimal cell models

NH Wubshet, AP Liu - Computational and Structural Biotechnology Journal, 2023 - Elsevier
Cells shield organelles and the cytosol via an active boundary predominantly made of
phospholipids and membrane proteins, yet allowing communication between the …

Studying actin-induced cell shape changes using Giant Unilamellar Vesicles and reconstituted actin networks

R Lopes dos Santos, C Campillo - Biochemical Society …, 2022 - portlandpress.com
Cell shape changes that are fuelled by the dynamics of the actomyosin cytoskeleton control
cellular processes such as motility and division. However, the mechanisms of interplay …

[HTML][HTML] Differential regulation of GUV mechanics via actin network architectures

NH Wubshet, B Wu, S Veerapaneni, AP Liu - Biophysical Journal, 2023 - cell.com
Actin networks polymerize and depolymerize to construct highly organized structures,
thereby endowing the mechanical phenotypes found in a cell. It is generally believed that …

Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture

Y Bashirzadeh, SA Redford, C Lorpaiboon… - Communications …, 2021 - nature.com
The proteins that make up the actin cytoskeleton can self-assemble into a variety of
structures. In vitro experiments and coarse-grained simulations have shown that the actin …

Rapid encapsulation of reconstituted cytoskeleton inside giant unilamellar vesicles

Y Bashirzadeh, N Wubshet, T Litschel… - Journal of …, 2021 - pmc.ncbi.nlm.nih.gov
Giant unilamellar vesicles (GUVs) are frequently used as models of biological membranes
and thus are a great tool to study membrane-related cellular processes in vitro. In recent …