Metabolites, substrates and substrate impurities may be toxic to cells by damaging biological
molecules, organelles, membranes or disrupting biological processes. Chemical stress is …

The sustainable production of biofuels will require the efficient utilization of lignocellulosic
biomass. A key barrier involves the creation of growth-inhibitory compounds by chemical …

A comparative transcriptome profiling between a riboflavin-producing Bacillus subtilis strain
RH33 and the wild-type strain B. subtilis 168 was performed, complemented with metabolite …

The objectives of this study were to improve understanding of the biochemical mechanisms
underlying the growth defects resulting from deletion of pflB and ldhA in E. coli (strain …

Deep mutational scanning can provide significant insights into the function of essential
genes in bacteria. Here, we developed a high‐throughput method for mutating essential …

Our limited ability to predict genotype–phenotype relationships has called for strategies that
allow testing of thousands of hypotheses in parallel. Deep scanning mutagenesis has been …

Engineering organisms for improved performance using lignocellulose feedstocks is an
important step towards a sustainable fuel and chemical industry. Cellulosic feedstocks …

The identification of relevant gene targets for engineering a desired trait is a key step in
combinatorial strain engineering. Here, we applied the multi-Scalar Analysis of Library …

Advances in genomics have improved the ability to map complex genotype-to-phenotype
relationships, like those required for engineering chemical tolerance. Here, we have applied …

The evolution of antibiotic resistance has engendered an impending global health crisis that
necessitates a greater understanding of how resistance emerges. The impact of nongenetic …