Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs

M Yeste - Theriogenology, 2016 - Elsevier
Cryopreservation is the most efficient method for long-term preservation of mammalian
sperm. However, freeze-thawing procedures may strongly impair the sperm function and …

Antioxidants and male fertility: from molecular studies to clinical evidence

D Martin-Hidalgo, MJ Bragado, AR Batista, PF Oliveira… - Antioxidants, 2019 - mdpi.com
Spermatozoa are physiologically exposed to reactive oxygen species (ROS) that play a
pivotal role on several sperm functions through activation of different intracellular …

Effects of reactive oxygen species on sperm function

HD Guthrie, GR Welch - Theriogenology, 2012 - Elsevier
Reactive oxygen species (ROS) formation and membrane lipid peroxidation have been
recognized as problems for sperm survival and fertility. The precise roles and detection of …

Determination of intracellular reactive oxygen species and high mitochondrial membrane potential in Percoll-treated viable boar sperm using fluorescence-activated …

HD Guthrie, GR Welch - Journal of animal science, 2006 - academic.oup.com
The use of frozen semen in the swine industry is limited by problems with viability and fertility
compared with liquid semen. Part of the reduction in sperm motility and fertility associated …

Artificial insemination with frozen‐thawed boar sperm

M Yeste, JE Rodríguez‐Gil… - Molecular reproduction …, 2017 - Wiley Online Library
Artificial insemination with frozen‐thawed semen in pigs is not a routine technique; its use is
restricted to specific cases, such as preservation of valuable genetic material (germplasm …

Recent advances in boar sperm cryopreservation: state of the art and current perspectives

M Yeste - Reproduction in domestic animals, 2015 - Wiley Online Library
Contents While sperm cryopreservation is the best technology to store boar semen for long‐
term periods, only 1% of all artificial inseminations (AI) conducted worldwide are made using …

Antioxidative effects of melatonin on kinetics, microscopic and oxidative parameters of cryopreserved bull spermatozoa

I Ashrafi, H Kohram, FF Ardabili - Animal Reproduction Science, 2013 - Elsevier
Reactive oxygen species generated during the freeze–thawing process may reduce sperm
quality. This study evaluates the effects of melatonin supplementation as an antioxidant in …

Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase

J Roca, MJ Rodríguez, MA Gil, G Carvajal… - Journal of …, 2005 - Wiley Online Library
In the present study the potential benefit of reactive oxygen species (ROS)‐scavenging
enzymes superoxide dismutase (SOD) and catalase (CAT) when cryopreserving boar …

Effect of α-tocopherol supplementation during boar semen cryopreservation on sperm characteristics and expression of apoptosis related genes

YJ Jeong, MK Kim, HJ Song, EJ Kang, SA Ock… - Cryobiology, 2009 - Elsevier
Boar semen is extremely vulnerable to cold shock and sensitive to peroxidative damage due
to high content of unsaturated fatty acids in the phospholipids of the plasma membrane and …

Anti-oxidant supplementation improves boar sperm characteristics and fertility after cryopreservation: comparison between cysteine and rosemary (Rosmarinus …

C Malo, L Gil, N Gonzalez, F Martínez, R Cano… - Cryobiology, 2010 - Elsevier
Anti-oxidants partially ameliorated the detrimental effects of reactive oxidative substances
produced during cryopreservation. The objective of the study was to determine the effect of …