Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …
Best practices for aggregate quantitation of antibody therapeutics by sedimentation velocity analytical ultracentrifugation
GM Bou-Assaf, IL Budyak, M Brenowitz, ES Day… - Journal of …, 2022 - Elsevier
Analytical ultracentrifugation (AUC) is a critical analytical tool supporting the development
and manufacture of protein therapeutics. AUC is routinely used as an assay orthogonal to …
and manufacture of protein therapeutics. AUC is routinely used as an assay orthogonal to …
Anion-exchange HPLC assay for separation and quantification of empty and full capsids in multiple adeno-associated virus serotypes
SL Khatwani, A Pavlova, Z Pirot - Molecular Therapy Methods & Clinical …, 2021 - cell.com
Gene therapy has entered a new era where numerous therapies for severe and rare
diseases are generating robust and compelling clinical results. The rapid improvements in …
diseases are generating robust and compelling clinical results. The rapid improvements in …
AAV analysis by sedimentation velocity analytical ultracentrifugation: beyond empty and full capsids
AE Yarawsky, V Zai-Rose, HM Cunningham… - European Biophysics …, 2023 - Springer
The recent surge of therapeutic interest in recombinant adeno-associated viral (AAV) vectors
for targeted DNA delivery has brought analytical ultracentrifugation (AUC) into the spotlight …
for targeted DNA delivery has brought analytical ultracentrifugation (AUC) into the spotlight …
Development of a two-dimensional liquid chromatography-mass spectrometry platform for simultaneous multi-attribute characterization of adeno-associated viruses
Adeno-associated viruses (AAVs) are non-enveloped, single-stranded DNA viruses that
have recently emerged as an attractive vector for delivering genetic materials to hosts for …
have recently emerged as an attractive vector for delivering genetic materials to hosts for …
Reincarnation of the analytical ultracentrifuge: Emerging opportunities for nanomedicine
OJ Valderrama, I Nischang - 2021 - ACS Publications
The analytical ultracentrifuge (AUC) and the modern field of analytical ultracentrifugation
found its inception approximately a century ago. We highlight the scope of its major …
found its inception approximately a century ago. We highlight the scope of its major …
DNA supercoiling-induced shapes alter minicircle hydrodynamic properties
R Waszkiewicz, M Ranasinghe, JM Fogg… - Nucleic acids …, 2023 - academic.oup.com
DNA in cells is organized in negatively supercoiled loops. The resulting torsional and
bending strain allows DNA to adopt a surprisingly wide variety of 3-D shapes. This interplay …
bending strain allows DNA to adopt a surprisingly wide variety of 3-D shapes. This interplay …
An automated interface for sedimentation velocity analysis in SEDFIT
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is an indispensable tool for
the study of particle size distributions in biopharmaceutical industry, for example, to …
the study of particle size distributions in biopharmaceutical industry, for example, to …
Analytical band centrifugation for the separation and quantification of empty and full AAV particles
H Khasa, G Kilby, X Chen, C Wang - Molecular Therapy Methods & Clinical …, 2021 - cell.com
Analytical band centrifugation (ABC) was first developed for the separation of
macromolecules in centrifugation cells~ 60 years ago. Since its development, ABC has been …
macromolecules in centrifugation cells~ 60 years ago. Since its development, ABC has been …
Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan
Aim: We present multi-wavelength (MW) analytical ultracentrifugation (AUC) methods
offering superior accuracy for adeno-associated virus characterization and quantification …
offering superior accuracy for adeno-associated virus characterization and quantification …