The design of enzyme catalytic stability is of great significance in medicine and industry.
However, traditional methods are time-consuming and costly. Hence, a growing number of …

Progress in technology and algorithms throughout the past decade has transformed the field
of protein design and engineering. Computational approaches have become well-engrained …

Thermostable proteins find their use in numerous biomedical and biotechnological
applications. However, the computational design of stable proteins often results in single …

Directed protein evolution applies repeated rounds of genetic mutagenesis and phenotypic
screening and is often limited by experimental throughput. Through in silico prioritization of …

Highlights•Enzymes are increasingly used in technological applications but often require
prior optimization.•Computational tools save time and effort in protein engineering …

A very large number of computational methods to predict the change in thermodynamic
stability of proteins due to mutations have been developed during the last 30 years, and …

Structural information can help engineer enzymes. Usually, specific amino acids in particular
regions are targeted for functional reconstruction to enhance the catalytic performance …

Most amino acid substitutions in a protein either lead to partial loss-of-function or are near
neutral. Several studies have shown the existence of second-site mutations that can rescue …

Understanding the details of a protein folding mechanism can be a challenging and complex
task. One system with an interesting folding behavior is the α-spectrin domain, where the …

Implementing hyperthermostable carbonic anhydrases into CO 2 capture and storage
technologies in order to increase the rate of CO 2 absorption from the industrial flue gases is …