The hallmarks of living systems: towards creating artificial cells

NA Yewdall, AF Mason, JCM Van Hest - Interface Focus, 2018 - royalsocietypublishing.org
Despite the astonishing diversity and complexity of living systems, they all share five
common hallmarks: compartmentalization, growth and division, information processing …

Encapsulation of the cytoskeleton: towards mimicking the mechanics of a cell

Y Bashirzadeh, AP Liu - Soft Matter, 2019 - pubs.rsc.org
The cytoskeleton of a cell controls all the aspects of cell shape changes and motility from its
physiological functions for survival to reproduction to death. The structure and dynamics of …

Optimized cDICE for efficient reconstitution of biological systems in giant unilamellar vesicles

L Van de Cauter, F Fanalista, L Van Buren… - ACS Synthetic …, 2021 - ACS Publications
Giant unilamellar vesicles (GUVs) are often used to mimic biological membranes in
reconstitution experiments. They are also widely used in research on synthetic cells, as they …

Actomyosin-driven division of a synthetic cell

L Baldauf, L Van Buren, F Fanalista… - ACS Synthetic …, 2022 - ACS Publications
One of the major challenges of bottom-up synthetic biology is rebuilding a minimal cell
division machinery. From a reconstitution perspective, the animal cell division apparatus is …

The new age of cell-free biology

V Noireaux, AP Liu - Annual review of biomedical engineering, 2020 - annualreviews.org
The cell-free molecular synthesis of biochemical systems is a rapidly growing field of
research. Advances in the Human Genome Project, DNA synthesis, and other technologies …

Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model

R Sakamoto, MP Murrell - Communications Biology, 2024 - nature.com
The architecture of the actin cortex determines the generation and transmission of stresses,
during key events from cell division to migration. However, its impact on myosin-induced cell …

Encapsulated actomyosin patterns drive cell-like membrane shape changes

Y Bashirzadeh, H Moghimianavval, AP Liu - Iscience, 2022 - cell.com
Cell shape changes from locomotion to cytokinesis are, to a large extent, driven by myosin-
driven remodeling of cortical actin patterns. Passive crosslinkers such as α-actinin and …

Engineering tissue-scale properties with synthetic cells: Forging one from many

AJ Lin, AZ Sihorwala, B Belardi - ACS Synthetic Biology, 2023 - ACS Publications
In metazoans, living cells achieve capabilities beyond individual cell functionality by
assembling into multicellular tissue structures. These higher-order structures represent …

Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex

R Sakamoto, DS Banerjee, V Yadav, S Chen… - Communications …, 2023 - nature.com
The accumulation and transmission of mechanical stresses in the cell cortex and membrane
determines the mechanics of cell shape and coordinates essential physical behaviors, from …

Artificial cells for in vivo biomedical applications through red blood cell biomimicry

J Waeterschoot, W Gosselé, Š Lemež… - Nature …, 2024 - nature.com
Recent research in artificial cell production holds promise for the development of delivery
agents with therapeutic effects akin to real cells. To succeed in these applications, these …