Fluorescence lifetime imaging–techniques and applications

W Becker - Journal of microscopy, 2012 - Wiley Online Library
Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments

K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Spectral variation of fluorescence lifetime near single metal nanoparticles

J Li, AV Krasavin, L Webster, P Segovia, AV Zayats… - Scientific reports, 2016 - nature.com
We explore the spectral dependence of fluorescence enhancement and the associated
lifetime modification of fluorescent molecules coupled to single metal nanoparticles …

Delayed photoluminescence in metal-conjugated fluorophores

M Yang, P Moroz, Z Jin, DS Budkina… - Journal of the …, 2019 - ACS Publications
Assemblies of metal nanostructures and fluorescent molecules represent a promising
platform for the development of biosensing and near-field imaging applications. Typically …

A whole-body dual-modality radionuclide optical strategy for preclinical imaging of metastasis and heterogeneous treatment response in different microenvironments

GO Fruhwirth, S Diocou, PJ Blower, T Ng… - Journal of Nuclear …, 2014 - Soc Nuclear Med
Imaging spontaneous cancer cell metastasis or heterogeneous tumor responses to drug
treatment in vivo is difficult to achieve. The goal was to develop a new highly sensitive and …

Resonant electro-optic imaging for microscopy at nanosecond resolution

AJ Bowman, MA Kasevich - ACS nano, 2021 - ACS Publications
We demonstrate an electro-optic wide-field method to enable fluorescence lifetime
microscopy (FLIM) with high throughput and single-molecule sensitivity. Resonantly driven …

How Förster resonance energy transfer imaging improves the understanding of protein interaction networks in cancer biology

GO Fruhwirth, LP Fernandes, G Weitsman… - …, 2011 - Wiley Online Library
Herein we discuss how FRET imaging can contribute at various stages to delineate the
function of the proteome. Therefore, we briefly describe FRET imaging techniques, the …

Morphology and composition of insoluble Brown carbon from biomass burning

P Gao, C Zhou, C Lian, X Wang… - ACS Earth and Space …, 2022 - ACS Publications
Biomass burning (BB) is a primary source of brown carbon (BrC) in the atmosphere. In the
present work, for the first time, the application of an impinger for BrC collection was …

CXCR4 chemokine receptor antagonists: nickel (II) complexes of configurationally restricted macrocycles

R Smith, D Huskens, D Daelemans, RE Mewis… - Dalton …, 2012 - pubs.rsc.org
Tetraazamacrocyclic complexes of transition metals provide useful units for incorporating
multiple coordination interactions into a single protein binding molecule. They can be …

A multi-functional imaging approach to high-content protein interaction screening

DR Matthews, GO Fruhwirth, G Weitsman, LM Carlin… - PloS one, 2012 - journals.plos.org
Functional imaging can provide a level of quantification that is not possible in what might be
termed traditional high-content screening. This is due to the fact that the current state-of-the …