In a plant-specific mode of protein glycosylation, various sugars and glycans are attached to
hydroxyproline giving rise to a variety of diverse O-glycoproteins. The sub-family of …

Small molecule inhibitors of glycosylation enzymes are valuable tools for dissecting glycan
functions and potential drug candidates. Screening for inhibitors of glycosyltransferases are …

The complex multi-step process of glycosylation occurs in a single cell, yet current analytics
generally cannot measure the output (the glycome) of a single cell. Here, we addressed this …

Abstract Polypeptide GalNAc-transferase T3 (GalNAc-T3) regulates fibroblast growth factor
23 (FGF23) by O-glycosylating Thr178 in a furin proprotein processing motif RHT178R↓ S …

Highlights•Review the challenges to decipher the glycocode and strategies to overcome
these.•Tools and repositories for investigating the protein-carbohydrate complexes.• …

C1GalT1 is an essential inverting glycosyltransferase responsible for synthesizing the core 1
structure, a common precursor for mucin-type O-glycans found in many glycoproteins. To …

Golgi membrane proteins such as glycosyltransferases and other glycan-modifying enzymes
are key to glycosylation of proteins and lipids. Secretion of soluble Golgi enzymes that are …

Exploring the biological functions of the human glycome is highly challenging given its
tremendous structural diversity. We have developed stable libraries of isogenic HEK293 …

Numerous Golgi-resident enzymes implicated in glycosylation are regulated by the
conserved intramembrane protease SPPL3. SPPL3-catalyzed endoproteolysis separates …

Podocalyxin (Podxl) is a CD34-related cell surface sialomucin that is normally highly
expressed by adult vascular endothelia and kidney podocytes where it plays a key role in …