Traditional biochemical methods for enzyme detection are mainly based on antibody-based
immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in …

Leucine aminopeptidase (LAP) is one of important proteolytic enzymes and closely related
with pathogenesis of cancer and liver injury. Determination of LAP activity in serum is used …

Viruses that are typically benign sometimes invade the brainstem in otherwise healthy
children. We report bi-allelic DBR1 mutations in unrelated patients from different ethnicities …

The majority of genic transcription is intronic. Introns are removed by splicing as branched
lariat RNAs which require rapid recycling. The branch site is recognized during splicing …

In eukaryotic cells, intron lariats produced by the spliceosome contain a 2′ 5′
phosphodiester linkage. The RNA lariat debranching enzyme, Dbr1, is the only enzyme …

In eukaryotic cells, the introns are excised from pre-mRNA by the spliceosome. These
introns typically have a lariat configuration due to the 2′-5′ phosphodiester bond between …

The RNA lariat debranching enzyme is the sole enzyme responsible for hydrolyzing the 2′-
5′ phosphodiester bond in RNA lariats produced by the spliceosome. Here, we test the …

Abstract tRNA splicing endonuclease (TSEN) has a well-characterized role in transfer RNA
(tRNA) splicing but also other functions. For yeast TSEN, these other functions include …

The protein arginine methyltransferase 5 (PRMT5) is frequently overexpressed in human
cancer whose elevated activity is associated with poor clinical prognosis. Pharmacologic …

Lariat introns are generated during pre-mRNA splicing. The 2'-OH of the branch point
residue is linked to the 5'-terminus of the intron through a 2'-5'-phosphodiester bond. The 2' …