Application of PCR‐ELISA in molecular diagnosis

MJ Sue, SK Yeap, AR Omar… - BioMed research …, 2014 - Wiley Online Library
Polymerase chain reaction‐enzyme linked immunosorbent assay (PCR‐ELISA) is an
immunodetection method that can quantify PCR product directly after immobilization of …

[HTML][HTML] Detection of E. coli O157: H7 and Shigella dysenteriae toxins in clinical samples by PCR-ELISA

J Amani, A Ahmadpour, AAI Fooladi… - Brazilian Journal of …, 2015 - SciELO Brasil
Shiga toxin producing bacteria are potential causes of serious human disease such as
hemorrhagic colitis, severe inflammations of ileocolonic regions of gastrointestinal tract …

Multidrug-resistant tuberculosis

DA Wulandari, YW Hartati, AU Ibrahim, DAE Pitaloka - Clinica Chimica …, 2024 - Elsevier
One of predominant contributors to global mortality is tuberculosis (TB), an infection caused
by Mycobacterium tuberculosis (MTB). Inappropriate and ineffectual treatment can lead to …

A rapid and sensitive diagnosis of typhoid fever based on nested PCR-voltammetric DNA biosensor using flagellin gene fragment

YW Hartati, S Wyantuti, ML Firdaus… - Indonesian Journal of …, 2016 - journal.ugm.ac.id
Typhoid fever caused by Salmonella typhi is an important issue for public health in the
world. Laboratory methods for rapid and sensitive diagnosis are very important for disease …

Novel PCR-ELISA technique as a good substitute in molecular assay

F Tayebeh, S Nazarian, SA Mirhosseini… - Journal of Applied …, 2017 - eprints.bmsu.ac.ir
Due to the spread of infectious diseases, the existence of a rapid and sensitive detection
method is necessary today. Polymerase chain reaction-enzyme linked immunosorbent …

Rapid and Specific Detection of Salmonella typhimurium by PCR-ELISA

H Ardestani, SL Mousavi Gargari… - Pathobiology …, 2008 - mjms.modares.ac.ir
Objectives: Salmonella typhimurium is important food-borne pathogen responsible for
gastroenteritis. In this work a polymerase chain reaction based enzyme linked …

[HTML][HTML] Detection of Shigella dysenteriae STX1 gene from Mazandaran province clinical samples by PCR-ELISA method

A Ahmadpour, E Fatahi, A Jafar… - Iranian Journal of …, 2016 - ijmm.ir
Background: Among enterobacteriaceae bacteria, Shigella dysenteriae produce a shiga
protein toxin, and have cytotoxicity, enterotoxin and neurotoxin activity. The toxin causes …

Species Specific PCR for Detection of salmonella typhi in Some Samples of Food

KI Hassan - platform.almanhal.com
The availability of reliable, rapid, and test systems for determination of the presence or
absence of food-borne pathogens has become increasingly important for the agricultural …

[PDF][PDF] MOLECULAR CHARACTERIZATION OF MALAYSIAN SWIFTLET SPECIES AND DEVELOPMENT OF PCR-ELISA METHOD FOR RAPID IDENTIFICATION OF …

S JEAN - 2016 - psasir.upm.edu.my
Edible birds‟ nest (EBN) is a precious functional food that has been used for several
hundred years by Chinese communities around the world (Lin et al., 2009). EBN is mainly …

[PDF][PDF] Detection of stx1 gene in Shigella dysenteriae from Mazandaran province clinical samples by PCR-ELISA method

A Ahmadpour, E Fatahi, J Amani, AAI Fooladi… - 2016 - ijmm.ir
Materials and Methods: The stx1 sequence (490bp) as a target gene was amplified by Dig-
dUTP labeled, then product was coating on microplate and detection was done using an …