A guide to molecular and functional investigations of platelets to bridge basic and clinical sciences

T Tyagi, K Jain, SX Gu, M Qiu, VW Gu… - Nature cardiovascular …, 2022 - nature.com
Platelets have been shown to be associated with pathophysiological processes beyond
thrombosis, demonstrating critical additional roles in homeostatic processes, such as …

Viscoelastic hemostatic assays: moving from the laboratory to the site of care—a review of established and emerging technologies

J Hartmann, M Murphy, JD Dias - Diagnostics, 2020 - mdpi.com
Viscoelastic-based techniques to evaluate whole blood hemostasis have advanced
substantially since they were first developed over 70 years ago but are still based upon the …

Live-cell super-resolved PAINT imaging of piconewton cellular traction forces

JM Brockman, H Su, AT Blanchard, Y Duan, T Meyer… - Nature …, 2020 - nature.com
Despite the vital role of mechanical forces in biology, it still remains a challenge to image
cellular force with sub-100-nm resolution. Here, we present tension points accumulation for …

Transcatheter aortic valve implantation represents an anti-inflammatory therapy via reduction of shear stress–induced, piezo-1–mediated monocyte activation

S Baratchi, MTK Zaldivia, M Wallert, J Loseff-Silver… - Circulation, 2020 - Am Heart Assoc
Background: Aortic valve stenosis is an increasingly prevalent degenerative and
inflammatory disease. Transcatheter aortic valve implantation (TAVI) has revolutionized its …

Detection of cellular traction forces via the force-triggered Cas12a-mediated catalytic cleavage of a fluorogenic reporter strand

Y Duan, F Szlam, Y Hu, W Chen, R Li, Y Ke… - Nature Biomedical …, 2023 - nature.com
Molecular forces generated by cell receptors are infrequent and transient, and hence difficult
to detect. Here we report an assay that leverages the CRISPR-associated protein 12a …

Mechanically triggered hybridization chain reaction

Y Duan, R Glazier, A Bazrafshan, Y Hu… - Angewandte Chemie …, 2021 - Wiley Online Library
Cells transmit piconewton forces to receptors to mediate processes such as migration and
immune recognition. A major challenge in quantifying such forces is the sparsity of cell …

Reduced platelet forces underlie impaired hemostasis in mouse models of MYH9-related disease

J Baumann, L Sachs, O Otto, I Schoen, P Nestler… - Science …, 2022 - science.org
MYH9-related disease patients with mutations in the contractile protein nonmuscle myosin
heavy chain IIA display, among others, macrothrombocytopenia and a mild-to-moderate …

Hemodynamic analysis for stenosis microfluidic model of thrombosis with refined computational fluid dynamics simulation

YC Zhao, P Vatankhah, T Goh, R Michelis, K Kyanian… - Scientific reports, 2021 - nature.com
Disturbed blood flow has been increasingly recognized for its critical role in platelet
aggregation and thrombosis. Microfluidics with hump shaped contractions have been …

Novel Movable Typing for Personalized Vein‐Chips in Large Scale: Recapitulate Patient‐Specific Virchow's Triad and its Contribution to Cerebral Venous Sinus …

YC Zhao, Y Zhang, Z Wang, F Jiang… - Advanced Functional …, 2023 - Wiley Online Library
Abstract The Vein‐Chip recapitulates CVST Virchow's triad and enables systematic
characterization of venous thrombogenesis with respect to fibrin formation and platelet …

Alterations in platelet behavior after major trauma: adaptive or maladaptive?

P Vulliamy, LZ Kornblith, ME Kutcher, MJ Cohen… - Platelets, 2021 - Taylor & Francis
Platelets are damage sentinels of the intravascular compartment, initiating and coordinating
the primary response to tissue injury. Severe trauma and hemorrhage induce profound …