[HTML][HTML] Membrane models and experiments suitable for studies of the cholesterol bilayer domains

I Mardešić, Z Boban, WK Subczynski, M Raguz - Membranes, 2023 - mdpi.com
Cholesterol (Chol) is an essential component of animal cell membranes and is most
abundant in plasma membranes (PMs) where its concentration typically ranges from 10 to …

[HTML][HTML] Alveolar dynamics and beyond–the importance of surfactant protein C and cholesterol in lung homeostasis and fibrosis

K Sehlmeyer, J Ruwisch, N Roldan… - Frontiers in …, 2020 - frontiersin.org
Surfactant protein C (SP-C) is an important player in enhancing the interfacial adsorption of
lung surfactant lipid films to the alveolar air-liquid interface. Doing so, surface tension drops …

Functions of cholesterol and the cholesterol bilayer domain specific to the fiber-cell plasma membrane of the eye lens

WK Subczynski, M Raguz, J Widomska… - The Journal of …, 2012 - Springer
The most unique feature of the eye lens fiber-cell plasma membrane is its extremely high
cholesterol content. Cholesterol saturates the bulk phospholipid bilayer and induces …

Changes in the properties and organization of human lens lipid membranes occurring with age

L Mainali, M Raguz, WJ O'Brien… - Current eye …, 2017 - Taylor & Francis
Purpose: This research was undertaken to document the changes in the organization and
properties of human lens lipid membranes that occur with age. Methods: Human lens lipid …

Physical properties of lipid bilayers from EPR spin labeling and their influence on chemical reactions in a membrane environment

WK Subczynski, J Widomska, JB Feix - Free Radical Biology and Medicine, 2009 - Elsevier
The influence of a variety of microenvironmental factors on the inherent reactivity of
membrane-located reagents is poorly understood. A goal of this review is to provide detailed …

Using spin-label electron paramagnetic resonance (EPR) to discriminate and characterize the cholesterol bilayer domain

M Raguz, L Mainali, J Widomska… - Chemistry and physics of …, 2011 - Elsevier
Electron paramagnetic resonance (EPR) spin-labeling methods make it possible not only to
discriminate the cholesterol bilayer domain (CBD) but also to obtain information about the …

Properties of membranes derived from the total lipids extracted from the human lens cortex and nucleus

L Mainali, M Raguz, WJ O'Brien, WK Subczynski - Biophysical Journal, 2013 - cell.com
The organization and physical properties of lens lipid membranes made from the total lipids
extracted from the human lens cortex and nucleus of 41-to 60-year-old donors were …

Formation of cholesterol bilayer domains precedes formation of cholesterol crystals in cholesterol/dimyristoylphosphatidylcholine membranes: EPR and DSC studies

L Mainali, M Raguz, WK Subczynski - The Journal of Physical …, 2013 - ACS Publications
Saturation-recovery EPR along with DSC were used to determine the cholesterol content at
which pure cholesterol bilayer domains (CBDs) and cholesterol crystals begin to form in …

[HTML][HTML] Transbilayer organization of membrane cholesterol at low concentrations: implications in health and disease

A Chaudhuri, A Chattopadhyay - Biochimica et Biophysica Acta (BBA) …, 2011 - Elsevier
Cholesterol is an essential and representative lipid in higher eukaryotic cellular membranes
and is often found distributed nonrandomly in domains in biological membranes. A large …

Properties of membranes derived from the total lipids extracted from clear and cataractous lenses of 61–70-year-old human donors

L Mainali, M Raguz, WJ O'Brien… - European Biophysics …, 2015 - Springer
Human lens-lipid membranes prepared from the total lipids extracted from clear and
cataractous lens cortexes and nuclei of 61–70-year-old donors by use of a rapid solvent …