A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate,
comprising the interstitial introduction of an isolated polynucleotide into a tissue of the …

The difficulty of transfecting primary macrophages and macrophage ceil lines has meant that
relatively few studies on regulation of gene expression have been performed in these cells …

The migratory properties of memory T cells provide a model vector system for site-specific
delivery of therapeutic transgene factors to autoimmune inflammatory lesions. Lymph node …

Prior genetic and physical mapping has shown that the Naip gene cluster on mouse
chromosome 13D1-D3 contains a gene, Lgn1, that is responsible for determining the …

Polybrene/DMSO-assisted gene transfer is a simple and versatile transfection strategy
capable of producing high numbers of stable transfectants from adherent monolayer cultures …

Availability of an efficient transfection protocol is the first determinant in success of gene
transferring studies in mammalian cells which is accomplished experimentally for every …

This is an introductory book intended for readers coming newly to the subject of animal cell
culture, whether students, graduates or technicians. Its aim is to explain the basic techniques …

A method for delivering an isolated polynucleotide to the interior of a cell in a vertebrate,
comprising the interstitial introduction of an isolated polynucleotide into a tissue of the …

Changes in the concentration of calcium ions ([Ca2+]) within cellular organelles play a
central role in controlling cellular function. We have engineered the Ca2+ sensitive …

The ability to transfect cells with native genometic DNA and engineered gene/vector
constructs has played a leading role in accelerating our understanding of gene regulation …