In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

[HTML][HTML] Dynamic nuclear polarization for sensitivity enhancement in modern solid-state NMR

ASL Thankamony, JJ Wittmann, M Kaushik… - Progress in nuclear …, 2017 - Elsevier
The field of dynamic nuclear polarization has undergone tremendous developments and
diversification since its inception more than 6 decades ago. In this review we provide an in …

Mass spectrometry-based protein footprinting for higher-order structure analysis: fundamentals and applications

XR Liu, MM Zhang, ML Gross - Chemical reviews, 2020 - ACS Publications
Proteins adopt different higher-order structures (HOS) to enable their unique biological
functions. Understanding the complexities of protein higher-order structures and dynamics …

Intrinsically disordered proteins and intrinsically disordered protein regions

CJ Oldfield, AK Dunker - Annual review of biochemistry, 2014 - annualreviews.org
Intrinsically disordered proteins (IDPs) and IDP regions fail to form a stable structure, yet
they exhibit biological activities. Their mobile flexibility and structural instability are encoded …

Intrinsically disordered proteins from A to Z

VN Uversky - The international journal of biochemistry & cell biology, 2011 - Elsevier
The ideas that proteins might possess specific functions without being uniquely folded into
rigid 3D-structures and that these floppy polypeptides might constitute a noticeable part of …

Nitroxide spin labels and EPR spectroscopy: A powerful association for protein dynamics studies

F Torricella, A Pierro, E Mileo, V Belle… - Biochimica et Biophysica …, 2021 - Elsevier
Abstract Site-Directed Spin Labelling (SDSL) technique is based on the attachment of a
paramagnetic label onto a specific position of a protein (or other bio-molecules) and the …

Applications of NMR spectroscopy to systems biochemistry

TWM Fan, AN Lane - Progress in nuclear magnetic resonance spectroscopy, 2016 - Elsevier
The past decades of advancements in NMR have made it a very powerful tool for metabolic
research. Despite its limitations in sensitivity relative to mass spectrometric techniques, NMR …

The unfoldomics decade: an update on intrinsically disordered proteins

AK Dunker, CJ Oldfield, J Meng, P Romero, JY Yang… - BMC genomics, 2008 - Springer
Background Our first predictor of protein disorder was published just over a decade ago in
the Proceedings of the IEEE International Conference on Neural Networks (Romero P …

Protein structure determination in living cells by in-cell NMR spectroscopy

D Sakakibara, A Sasaki, T Ikeya, J Hamatsu… - Nature, 2009 - nature.com
Investigating proteins 'at work'in a living environment at atomic resolution is a major goal of
molecular biology, which has not been achieved even though methods for the three …

[图书][B] Structure and function of intrinsically disordered proteins

P Tompa, A Fersht - 2009 - taylorfrancis.com
The existence and functioning of intrinsically disordered proteins (IDPs) challenge the
classical structure-function paradigm that equates function with a well-defined 3D structure …