Single-cell RNA genomics technologies are revolutionizing biomedical science by profiling
single cells with unprecedented resolution, providing fundamental insights into the role of …

Despite gene expression programs being notoriously complex, RNA abundance is usually
assumed as a proxy for transcriptional activity. Recently developed approaches, able to …

Metabolic labeling of RNA is a powerful technique for studying the temporal dynamics of
gene expression. Nucleotide conversion approaches greatly facilitate the generation of data …

Regulation of mRNA degradation is critical for a diverse array of cellular processes and
developmental cell fate decisions. Many methods for determining mRNA half-lives rely on …

The quantification of the kinetic rates of RNA synthesis, processing, and degradation are
largely based on the integrative analysis of total and nascent transcription, the latter being …

The coordinated action of transcriptional and post-transcriptional machineries shapes gene
expression programs at steady state and determines their concerted response to …

Codon optimality is a major determinant of mRNA translation and degradation rates.
However, whether and through which mechanisms its effects are regulated remains poorly …

Metabolic labeling of newly transcribed RNAs coupled with RNA-seq is being increasingly
used for genome-wide analysis of RNA dynamics. Methods including standard biochemical …

Discovered in the 1980s, small regulatory RNAs (sRNAs) are now considered key actors in
virtually all aspects of bacterial physiology and virulence. Together with transcriptional and …

Over the last two decades, the field of molecular biology has witnessed a revolution due to
the development of next generation sequencing (NGS) technologies. NGS enables …