Cellular signaling networks are the foundation which determines the fate and function of
cells as they respond to various cues and stimuli. The discovery of fluorescent proteins over …

Over the past 20 years, protein engineering has been extensively used to improve and
modify the fundamental properties of fluorescent proteins (FPs) with the goal of adapting …

Super-resolution microscopy has become an increasingly popular and robust tool across the
life sciences to study minute cellular structures and processes. However, with the increasing …

Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

G protein-coupled receptors (GPCRs) are important drug targets that mediate various
signaling pathways by activating G proteins and engaging β-arrestin proteins. Despite its …

Compartmentalized biochemical activities are essential to all cellular processes, but there is
no generalizable method to visualize dynamic protein activities in living cells at a resolution …

Many proteins can be split into fragments that spontaneously reassemble, without covalent
linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment …

Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time
monitoring of molecular dynamics in space and time, which are crucial for the proper …

Cell signaling networks are intricately regulated in time and space to determine the
responses and fates of cells to different cues. Genetically encodable fluorescent and …

Synergistic advances in fluorescent protein engineering and live‐cell imaging techniques in
recent years have fueled the concurrent development and application of genetically …