The hairpin ribozyme belongs to the family of small catalytic RNAs that cleave RNA
substrates in a reversible reaction that generates 2′, 3′-cyclic phosphate and 5 …

The finding that RNA can perform enzyme-like functions in cells overturned the view that
biological catalysis is the exclusive realm of protein enzymes. The list of naturally occurring …

Background: Ribozymes catalyze an important set of chemical transformations in
metabolism, and 'engineered'ribozymes have been made that catalyze a variety of …

In vitro transcription using bacteriophage RNA polymerases and linearised plasmid or
oligodeoxynucleotide templates has been used extensively to produce RNA for biochemical …

Any RNA, when in a complex with another oligoribonucleotide known as an external guide
sequence (EGS), can become a substrate for ribonuclease P. Simulation of evolution in vitro …

Nucleic acids are increasingly being considered for therapeutic uses, either to interfere with
the function of specific nucleic acids or to bind specific proteins. Three types of nucleic acid …

In vitro selection experiments have been used to isolate active variants of the 50 nt hairpin
catalytic RNA motif following randomization of individual ribozyme domains and intensive …

The hairpin ribozyme is an example of a small catalytic RNA which catalyses the
endonucleolytic transesterification of RNA in a highly sequence-specific manner. The …

In vitro selection can generate functional sequence variants of an RNA structural motif that
are useful for comparative analysis. The technique Is particularly valuable in cases where …

An in vitro selection procedure was used to select RNase P ribozyme variants that efficiently
cleaved the sequence of the mRNA encoding thymidine kinase of herpes simplex virus 1. Of …