[HTML][HTML] Advances and applications of capillary electromigration methods in the analysis of therapeutic and diagnostic recombinant proteins–A Review
H Stutz - Journal of Pharmaceutical and Biomedical Analysis, 2023 - Elsevier
This review provides a comprehensive overview of methodological advances and
applications of CE in the analysis and characterization of recombinant therapeutic and …
applications of CE in the analysis and characterization of recombinant therapeutic and …
Exploring Parametric and Mechanistic Differences between Expi293FTM and ExpiCHO-STM Cells for Transient Antibody Production Optimization
J Zhou, GG Yan, D Cluckey, C Meade, M Ruth, R Sorm… - Antibodies, 2023 - mdpi.com
Rapidly producing drug-like antibody therapeutics for lead molecule discovery and
candidate optimization is typically accomplished by large-scale transient gene expression …
candidate optimization is typically accomplished by large-scale transient gene expression …
Engineering Ag43 signal peptides with bacterial display and selection
Protein display, secretion, and export in prokaryotes are essential for utilizing microbial
systems as engineered living materials, medicines, biocatalysts, and protein factories. To …
systems as engineered living materials, medicines, biocatalysts, and protein factories. To …
More than meets the kappa for antibody Superantigen protein L (PpL)
Immunoglobulin superantigens play an important role in affinity purification of antibodies
and the microbiota-immune axis at mucosal areas. Based on current understanding …
and the microbiota-immune axis at mucosal areas. Based on current understanding …
Slight variations in the sequence downstream of the polyadenylation signal significantly increase transgene expression in HEK293T and CHO cells
Compared to transcription initiation, much less is known about transcription termination. In
particular, large-scale mutagenesis studies have, so far, primarily concentrated on promoter …
particular, large-scale mutagenesis studies have, so far, primarily concentrated on promoter …
Comparison of vector elements and process conditions in transient and stable suspension HEK293 platforms using SARS-CoV-2 receptor binding domain as a model …
EA Green, NK Hamaker, KH Lee - BMC biotechnology, 2023 - Springer
Background Mammalian cell lines are frequently used as protein expression hosts because
of their ability to correctly fold and assemble complex proteins, produce them at high titers …
of their ability to correctly fold and assemble complex proteins, produce them at high titers …
Variable-heavy (VH) families influencing IgA1&2 engagement to the antigen, FcαRI and superantigen proteins G, A, and L
Interest in IgA as an alternative antibody format has increased over the years with much
remaining to be investigated in relation to interactions with immune cells. Considering the …
remaining to be investigated in relation to interactions with immune cells. Considering the …
The influence of variable-heavy chain families on IgG2, 3, 4, FcγRs and B-cell superantigens protein G and L binding using biolayer interferometry
AM Deacy, SKE Gan - Antibody Therapeutics, 2023 - academic.oup.com
As the most abundant immunoglobulin in blood and the most common human isotype used
for therapeutic monoclonal antibodies, the engagement and activation of its Fc receptors by …
for therapeutic monoclonal antibodies, the engagement and activation of its Fc receptors by …
[HTML][HTML] The synergistic effects of the constant region and variable heavy chain families of multimeric immunoglobulin M on its interaction with Fc-mu receptor and …
Aim: As the primary response antibody with increasing use as a therapeutic immunoglobulin
(Ig) format, IgM is also the largest antibody structure among the five major human isotypes …
(Ig) format, IgM is also the largest antibody structure among the five major human isotypes …
The synergistic effects of the VH families and CH regions of multimeric IgM on its interaction with FcμR, and antigen
As the primary response antibody with increasing interest as a therapeutic antibody format,
IgM is also the largest antibody structure among the five major human isotypes …
IgM is also the largest antibody structure among the five major human isotypes …