1. INTRODUCTION The ability of amino acids to form a three-dimensional (3D) protein
structure is significantly interesting because it combines the aspects of biophysical …

Vaccines prevent infectious disease largely by inducing protective neutralizing antibodies
against vulnerable epitopes. Several major pathogens have resisted traditional vaccine …

Circular dichroism (CD) is an excellent tool for rapid determination of the secondary
structure and folding properties of proteins that have been obtained using recombinant …

Circular dichroism (CD) is an excellent spectroscopic technique for following the unfolding
and folding of proteins as a function of temperature. One of its principal applications is to …

It is well-known that the typical protein's three-dimensional structure is relatively unstable in
harsh conditions. A practical approach to maintain the folded state and thus improve the …

Conventional empirical methods for the quantification of the helical content of proteins in
solution using circular dichroism (CD) primarily rely on spectral data acquired between …

Differential scanning fluorimetry (DSF), also known as ThermoFluor or Thermal Shift Assay,
has become a commonly-used approach for detecting protein-ligand interactions …

Mancozeb is a broad-spectrum fungicide used extensively in agriculture to protect plants
from numerous diseases. Hemolysis of human erythrocytes on exposure to mancozeb has …

The present work aims to purify and perform a preliminary analysis on a thermostable serine
alkaline protease from a recently identified P. minor. The enzyme was purified 2.7-fold with a …

Abstract Reactive Red195 (RR195) was the first synthetic color patented for textile dying in
1856. Azo dyes have arisen considerable environmental concerns in recent decades due to …