To elucidate principles operating in native biological systems and to develop novel
biotechnologies, synthetic biology aims to build and integrate synthetic gene circuits within …

The rise of systems biology has ushered a new paradigm: the view of the cell as a system
that processes environmental inputs to drive phenotypic outputs. Synthetic biology provides …

Functional genomics efforts face tradeoffs between number of perturbations examined and
complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines …

NK-cell resistance to transduction is a major technical hurdle for developing NK-cell
immunotherapy. By using Baboon envelope pseudotyped lentiviral vectors (BaEV-LVs) …

Intercellular propagation of protein aggregation is emerging as a key mechanism in the
progression of several neurodegenerative diseases, including Alzheimer's disease and …

Chromatin boundary elements contribute to the partitioning of mammalian genomes into
topological domains to regulate gene expression. Certain boundary elements are adopted …

Understanding how viral and host factors interact and how perturbations impact infection is
the basis for designing antiviral interventions. Here we define the functional contribution of …

Synucleinopathies, including Parkinson's disease (PD), are associated with the misfolding
and mistrafficking of alpha-synuclein (α-syn). Here, using an ascorbate peroxidase (APEX) …

The targeted insertion and stable expression of a large genetic payload in primary human
cells demands methods that are robust, efficient and easy to implement. Large payload …

Background Macrophages have recently become attractive therapeutics in cancer
immunotherapy. The potential of macrophages to infiltrate and influence solid malignancies …