Aminoglycosides have been an essential component of the armamentarium in the treatment
of life-threatening infections. Unfortunately, their efficacy has been reduced by the surge and …

Plasmid‐mediated quinolone resistance (PMQR) was late in being discovered. Nalidixic
acid, the first quinolone to be used clinically, was introduced in 1967 for urinary tract …

In the past 2 decades, we have observed a rapid increase of infections due to multidrug-
resistant Enterobacteriaceae. Regrettably, these isolates possess genes encoding for …

Antimicrobial consumption is one of the major contributing factors facilitating the
development and maintenance of bacteria exhibiting antimicrobial resistance. Plasmid …

Plasmid-mediated quinolone resistance genes in clinical strains cannot be detected by
phenotypic traits but require gene detection. We developed a multiplex real-time polymerase …

Here we report a single nucleotide polymorphism (SNP) based genotyping method for
Klebsiella pneumoniae utilising high-resolution melting (HRM) analysis of fragments within …

Background. Routine medical microbiology diagnostics relies on conventional cultivation
followed by phenotypic techniques for identification of pathogenic bacteria and fungi. This is …

Abstract The aac (6')-Ib gene is the most prevalent gene that encodes aminoglycoside-
modifying enzymes and confers resistance to tobramycin, kanamycin, and amikacin. The …

We have developed a simple PCR-based high-resolution melt curve analysis for
identification of the quinolone resistance gene aac (6′)-Ib-cr through regions …

Diagnosis of invasive aspergillosis (IA) requires increasingly rapid molecular methods that
enable sensitive detection and discrimination between species. We designed and evaluated …