The Gateway® cloning system sets a new trend in molecular biology by addressing the
difficulties of adaptability, efficiency and compatibility of the traditional cloning approaches …

Salmonella develops into resident bacteria in epithelial cells, and the autophagic machinery
(Atg) is thought to play an important role in this process. In this paper, we show that an …

The increasing sophistication of synthetic biology is creating a demand for robust, broadly
accessible methodology for constructing multigene pathways inside of the cell. Due to the …

The production of cells capable of expressing gene (s) of interest is important for a variety of
applications in biomedicine and biotechnology, including gene therapy and animal …

It has previously been shown that the phage-derived phiC31 integrase can efficiently target
native pseudo-attachment sites in the genome of various species in cultured cells, as well as …

Previously, we found that Ureaplasma parvum internalised into HeLa cells and cytosolic
accumulation of galectin‐3. U. parvum induced the host cellular membrane damage and …

As molecular biology tasks progress from single gene expression to the implementation of
entire metabolic pathways and behavioral genetic circuitry, DNA assembly, the process of …

Genital mycoplasmas, including Ureaplasma spp., are among the smallest human
pathogenic bacteria and are associated with preterm birth. Electron microscopic observation …

Expression of multiple transgenes in cells or whole organisms is a powerful tool for basic
research of various biological functions and potentially for clinical applications such as gene …

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