Evaluation of X‐ray tomography contrast agents: A review of production, protocols, and biological applications

MM Koç, N Aslan, AP Kao… - Microscopy research and …, 2019 - Wiley Online Library
X‐ray computed tomography is a strong tool that finds many applications both in medical
applications and in the investigation of biological and nonbiological samples. In the clinics …

Three-dimensional visualisation of soft biological structures by X-ray computed micro-tomography

T Shearer, RS Bradley… - Journal of cell …, 2016 - journals.biologists.com
Whereas the two-dimensional (2D) visualisation of biological samples is routine, three-
dimensional (3D) imaging remains a time-consuming and relatively specialised pursuit …

Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues

A Maes, C Pestiaux, A Marino, T Balcaen… - Nature …, 2022 - nature.com
Biological tissues comprise a spatially complex structure, composition and organization at
the microscale, named the microstructure. Given the close structure-function relationships in …

Phosphotungstic acid‐enhanced microCT: Optimized protocols for embryonic and early postnatal mice

KM Lesciotto, SM Motch Perrine… - Developmental …, 2020 - Wiley Online Library
Background Given the need for descriptive and increasingly mechanistic morphological
analyses, contrast‐enhanced microcomputed tomography (microCT) represents perhaps the …

A quality optimization approach to image Achilles tendon microstructure by phase-contrast enhanced synchrotron micro-tomography

M Pierantoni, I Silva Barreto, M Hammerman… - Scientific reports, 2021 - nature.com
Achilles tendons are mechanosensitive, and their complex hierarchical structure is in part
the result of the mechanical stimulation conveyed by the muscles. To fully understand how …

[HTML][HTML] A new strain energy function for the hyperelastic modelling of ligaments and tendons based on fascicle microstructure

T Shearer - Journal of biomechanics, 2015 - Elsevier
A new strain energy function for the hyperelastic modelling of ligaments and tendons based
on the geometrical arrangement of their fibrils is derived. The distribution of the crimp angles …

[HTML][HTML] A practical guide for in situ mechanical testing of musculoskeletal tissues using synchrotron tomography

E Dall'Ara, AJ Bodey, H Isaksson, G Tozzi - journal of the mechanical …, 2022 - Elsevier
Musculoskeletal tissues are complex hierarchical materials where mechanical response is
linked to structural and material properties at different dimensional levels. Therefore, high …

Optimal contrast agent staining of ligaments and tendons for X-ray computed tomography

R Balint, T Lowe, T Shearer - PloS one, 2016 - journals.plos.org
X-ray computed tomography has become an important tool for studying the microstructures
of biological soft tissues, such as ligaments and tendons. Due to the low X-ray attenuation of …

[HTML][HTML] MicroCT optimisation for imaging fascicular anatomy in peripheral nerves

N Thompson, E Ravagli, S Mastitskaya… - Journal of neuroscience …, 2020 - Elsevier
Background Due to the lack of understanding of the fascicular organisation, vagus nerve
stimulation (VNS) leads to unwanted off-target effects. Micro-computed tomography …

The relative compliance of energy-storing tendons may be due to the helical fibril arrangement of their fascicles

T Shearer, CT Thorpe… - Journal of the Royal …, 2017 - royalsocietypublishing.org
A nonlinear elastic microstructural model is used to investigate the relationship between
structure and function in energy-storing and positional tendons. The model is used to fit …