Amplification-free CRISPR/Cas detection technology: challenges, strategies, and perspectives
Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …
Solid-state nanopore sensors
Nanopore-based sensors have established themselves as a prominent tool for solution-
based, single-molecule analysis of the key building blocks of life, including nucleic acids …
based, single-molecule analysis of the key building blocks of life, including nucleic acids …
Plasmonic tweezers: for nanoscale optical trapping and beyond
Optical tweezers and associated manipulation tools in the far field have had a major impact
on scientific and engineering research by offering precise manipulation of small objects …
on scientific and engineering research by offering precise manipulation of small objects …
Point-of-care CRISPR/Cas nucleic acid detection: Recent advances, challenges and opportunities
JE van Dongen, JTW Berendsen… - Biosensors and …, 2020 - Elsevier
With the trend of moving molecular tests from clinical laboratories to on-site testing, there is a
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
need for nucleic acid based diagnostic tools combining the sensitivity, specificity and …
The CRISPR–Cas toolbox for analytical and diagnostic assay development
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
associated (Cas) systems have revolutionized biological and biomedical sciences in many …
CRISPR technology incorporating amplification strategies: molecular assays for nucleic acids, proteins, and small molecules
W Feng, AM Newbigging, J Tao, Y Cao, H Peng… - Chemical …, 2021 - pubs.rsc.org
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-
associated (Cas) protein systems have transformed the field of genome editing and …
associated (Cas) protein systems have transformed the field of genome editing and …
CRISPR‐Cas biochemistry and CRISPR‐based molecular diagnostics
Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
disease diagnostics, pathogen surveillance, and many more. However, this method requires …
Clustered regularly interspaced short palindromic repeats/Cas9-mediated lateral flow nucleic acid assay
X Wang, E Xiong, T Tian, M Cheng, W Lin, H Wang… - ACS …, 2020 - ACS Publications
The lateral flow assay is one of the most convenient analytical techniques for analyzing the
immune response, but its applicability to precise genetic analyses is limited by the false …
immune response, but its applicability to precise genetic analyses is limited by the false …
Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device
T Tian, Z Qiu, Y Jiang, D Zhu, X Zhou - Biosensors and Bioelectronics, 2022 - Elsevier
Although CRISPR-Cas12a and CRISPR-Cas13a systems work individually effective on
gene detection, their multiplex detection capability is limited due to the lack of specific probe …
gene detection, their multiplex detection capability is limited due to the lack of specific probe …
CRISPR-based detection of SARS-CoV-2: A review from sample to result
The current pandemic of the 2019 novel coronavirus (COVID-19) caused by SARS-CoV-2
(severe acute respiratory syndrome coronavirus-2) has raised significant public health …
(severe acute respiratory syndrome coronavirus-2) has raised significant public health …