Advancing crop transformation in the era of genome editing

F Altpeter, NM Springer, LE Bartley, AE Blechl… - The Plant …, 2016 - academic.oup.com
Plant transformation has enabled fundamental insights into plant biology and revolutionized
commercial agriculture. Unfortunately, for most crops, transformation and regeneration …

[HTML][HTML] The CRISPR/Cas9 system for plant genome editing and beyond

L Bortesi, R Fischer - Biotechnology advances, 2015 - Elsevier
Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

Targeted, efficient sequence insertion and replacement in rice

Y Lu, Y Tian, R Shen, Q Yao, M Wang, M Chen… - Nature …, 2020 - nature.com
CRISPR–Cas9 methods have been applied to generate random insertions and deletions,
large deletions, targeted insertions or replacements of short sequences, and precise base …

Gene editing and crop improvement using CRISPR-Cas9 system

L Arora, A Narula - Frontiers in plant science, 2017 - frontiersin.org
Advancements in Genome editing technologies have revolutionized the fields of functional
genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

Principles, applications, and biosafety of plant genome editing using CRISPR-Cas9

K El-Mounadi, ML Morales-Floriano… - Frontiers in plant …, 2020 - frontiersin.org
The terms genome engineering, genome editing, and gene editing, refer to modifications
(insertions, deletions, substitutions) in the genome of a living organism. The most widely …

[HTML][HTML] Reduced enzymatic browning in potato tubers by specific editing of a polyphenol oxidase gene via ribonucleoprotein complexes delivery of the CRISPR/Cas9 …

MN González, GA Massa, M Andersson… - Frontiers in Plant …, 2020 - frontiersin.org
Polyphenol Oxidases (PPOs) catalyze the conversion of phenolic substrates to quinones,
leading to the formation of dark-colored precipitates in fruits and vegetables. This process …

CRISPR crops: plant genome editing toward disease resistance

T Langner, S Kamoun, K Belhaj - Annual review of …, 2018 - annualreviews.org
Genome editing by sequence-specific nucleases (SSNs) has revolutionized biology by
enabling targeted modifications of genomes. Although routine plant genome editing …

Towards CRISPR/Cas crops–bringing together genomics and genome editing

A Scheben, F Wolter, J Batley, H Puchta… - New …, 2017 - Wiley Online Library
With the rapid increase in the global population and the impact of climate change on
agriculture, there is a need for crops with higher yields and greater tolerance to abiotic …

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts

S Badhan, AS Ball, N Mantri - International Journal of Molecular Sciences, 2021 - mdpi.com
The current genome editing system Clustered Regularly Interspaced Short Palindromic
Repeats Cas9 (CRISPR/Cas9) has already confirmed its proficiency, adaptability, and …