An overview is presented of some of the major insights that have come from studies of the
structure, stability, and folding of T4 phage lysozyme. A major purpose of this review is to …

Selection of aptamers from nucleic acid libraries by in vitro evolution represents a powerful
method of identifying high-affinity ligands for a broad range of molecular targets …

Protein cavities and tunnels are critical in determining phenomena such as ligand binding,
molecular transport, and enzyme catalysis. Molecular dynamics (MD) simulations enable the …

We have set up and manually curated a dataset containing experimental information on the
impact of amino acid substitutions in a protein on its thermal stability. It consists of a …

This paper gives an overview of the application of high-pressure to study the
folding/unfolding processes of proteins using Ribonuclease A as a model protein. A …

Metal ions homeostasis plays an important role in biological processes. The ability to detect
the concentration of metal ions in biological fluids is often challenged by the obvious …

The total chemical synthesis of RNase A using modern chemical ligation methods is
described, illustrating the significant advances that have been made in chemical protein …

Conformational flexibility between structural ensembles is an essential component of
enzyme function. Although the broad dynamical landscape of proteins is known to promote a …

Changes in protein stability are commonly reported as changes in the melting temperature,
Δ TM, or as changes in unfolding free energy at a particular temperature, ΔΔ G°. Using data …

In this work, we applied the sequence-based statistical coupling analysis approach to
characterize conserved amino acid networks important for biochemical function in the …