Metabolic engineering has allowed the production of a diverse number of valuable
chemicals using microbial organisms. Many biological challenges for improving bio …

Highlights•Isoprenoids are industrially important natural products.•Yeast are key industrial
production vehicles.•New synthetic biology approaches in recent years are reviewed.•Future …

The design and optimization of biological systems is an inherently complex undertaking that
requires careful balancing of myriad synergistic and antagonistic variables. However …

Synthetic biology continues to progress by relying on more robust tools for transcriptional
control, of which promoters are the most fundamental component. Numerous studies have …

Traditional plastic products have caused serious environmental pollution due to difficulty to
be degraded in the natural environment. In the recent years, biodegradable plastics are …

Terpenoids, also called isoprenoids, are a large and highly diverse family of natural
products with important medical and industrial properties. However, a limited production of …

Yarrowia lipolytica is recognized as an excellent non-conventional yeast in the field of
biomanufacturing, where it is used as a host to produce oleochemicals, terpenes, organic …

Highlights•Engineered cell experiences metabolic imbalance with reduced fitness.•
Overproduction phenotype requires dynamic redistribution of cellular resource.•Metabolite …

Promoters play an essential role in the regulation of gene expression for fine-tuning genetic
circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However …

Saccharomyces cerevisiae is widely employed as a cell factory for the production of biofuels.
However, product toxicity has hindered improvements in biofuel production. Here, we …