Programmed cell death detection methods: a systematic review and a categorical comparison

S Kari, K Subramanian, IA Altomonte, A Murugesan… - Apoptosis, 2022 - Springer
Programmed cell death is considered a key player in a variety of cellular processes that
helps to regulate tissue growth, embryogenesis, cell turnover, immune response, and other …

Challenges of applying multicellular tumor spheroids in preclinical phase

SJ Han, S Kwon, KS Kim - Cancer cell international, 2021 - Springer
Abstract The three-dimensional (3D) multicellular tumor spheroids (MCTs) model is
becoming an essential tool in cancer research as it expresses an intermediate complexity …

The MTT assay: utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis

M Ghasemi, T Turnbull, S Sebastian… - International journal of …, 2021 - mdpi.com
The MTT assay for cellular metabolic activity is almost ubiquitous to studies of cell toxicity;
however, it is commonly applied and interpreted erroneously. We investigated the …

Guidelines for cell viability assays

S Kamiloglu, G Sari, T Ozdal, E Capanoglu - Food Frontiers, 2020 - Wiley Online Library
Recently, the interest in the application of cell viability assays has been increasing in various
fields. Cell viability assays may be broadly classified as (a) dye exclusion assays,(b) …

Validation of in-vitro bioassay methods: Application in herbal drug research

G Indrayanto, GS Putra, F Suhud - Profiles of drug substances, excipients …, 2021 - Elsevier
This present review described the validation method of in-vitro bioassay for its application in
herbal drug research. Seven sequencing steps that can be taken for performing a valid …

[HTML][HTML] SARS-coronavirus-2 replication in Vero E6 cells: replication kinetics, rapid adaptation and cytopathology

NS Ogando, TJ Dalebout… - Journal of General …, 2020 - microbiologyresearch.org
The sudden emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
at the end of 2019 from the Chinese province of Hubei and its subsequent pandemic spread …

Three-dimensional spheroids as in vitro preclinical models for cancer research

B Pinto, AC Henriques, PMA Silva, H Bousbaa - Pharmaceutics, 2020 - mdpi.com
Most cancer biologists still rely on conventional two-dimensional (2D) monolayer culture
techniques to test in vitro anti-tumor drugs prior to in vivo testing. However, the vast majority …

Stretchable, dynamic covalent polymers for soft, long-lived bioresorbable electronic stimulators designed to facilitate neuromuscular regeneration

YS Choi, YY Hsueh, J Koo, Q Yang, R Avila… - Nature …, 2020 - nature.com
Bioresorbable electronic stimulators are of rapidly growing interest as unusual therapeutic
platforms, ie, bioelectronic medicines, for treating disease states, accelerating wound …

Biosynthesis and characterization of gold nanoparticles using Brazilian red propolis and evaluation of its antimicrobial and anticancer activities

CEA Botteon, LB Silva, GV Ccana-Ccapatinta… - Scientific Reports, 2021 - nature.com
Gold nanoparticles (AuNPs) are highlighted due to their low toxicity, compatibility with the
human body, high surface area to volume ratio, and surfaces that can be easily modified …

Plant-based biosynthesis of copper/copper oxide nanoparticles: an update on their applications in biomedicine, mechanisms, and toxicity

D Letchumanan, SPM Sok, S Ibrahim, NH Nagoor… - Biomolecules, 2021 - mdpi.com
Plants are rich in phytoconstituent biomolecules that served as a good source of medicine.
More recently, they have been employed in synthesizing metal/metal oxide nanoparticles …