Current status and future perspectives on MRNA drug manufacturing
C Webb, S Ip, NV Bathula, P Popova… - Molecular …, 2022 - ACS Publications
The coronavirus disease of 2019 (COVID-19) pandemic launched an unprecedented global
effort to rapidly develop vaccines to stem the spread of the novel severe acute respiratory …
effort to rapidly develop vaccines to stem the spread of the novel severe acute respiratory …
Modified nucleic acids: replication, evolution, and next-generation therapeutics
K Duffy, S Arangundy-Franklin, P Holliger - Bmc Biology, 2020 - Springer
Modified nucleic acids, also called xeno nucleic acids (XNAs), offer a variety of advantages
for biotechnological applications and address some of the limitations of first-generation …
for biotechnological applications and address some of the limitations of first-generation …
Reversible methylation of m6Am in the 5′ cap controls mRNA stability
Internal bases in mRNA can be subjected to modifications that influence the fate of mRNA in
cells. One of the most prevalent modified bases is found at the 5′ end of mRNA, at the first …
cells. One of the most prevalent modified bases is found at the 5′ end of mRNA, at the first …
Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening
BT Porebski, M Balmforth, G Browne, A Riley… - Nature biomedical …, 2024 - nature.com
Developing therapeutic antibodies is laborious and costly. Here we report a method for
antibody discovery that leverages the Illumina HiSeq platform to, within 3 days, screen in the …
antibody discovery that leverages the Illumina HiSeq platform to, within 3 days, screen in the …
A two-residue nascent-strand steric gate controls synthesis of 2′-O-methyl- and 2′-O-(2-methoxyethyl)-RNA
Steric exclusion is a key element of enzyme substrate specificity, including in polymerases.
Such substrate specificity restricts the enzymatic synthesis of 2′-modified nucleic acids …
Such substrate specificity restricts the enzymatic synthesis of 2′-modified nucleic acids …
Bioorthogonal information storage in l-DNA with a high-fidelity mirror-image Pfu DNA polymerase
C Fan, Q Deng, TF Zhu - Nature Biotechnology, 2021 - nature.com
Natural DNA is exquisitely evolved to store genetic information. The chirally inverted l-DNA,
possessing the same informational capacity but resistant to biodegradation, may serve as a …
possessing the same informational capacity but resistant to biodegradation, may serve as a …
An RNA-cleaving threose nucleic acid enzyme capable of single point mutation discrimination
Y Wang, Y Wang, D Song, X Sun, Z Li, JY Chen… - Nature Chemistry, 2022 - nature.com
Threose nucleic acid has been considered a potential evolutionary progenitor of RNA
because of its chemical simplicity, base pairing properties and capacity for higher-order …
because of its chemical simplicity, base pairing properties and capacity for higher-order …
A general strategy for expanding polymerase function by droplet microfluidics
Polymerases that synthesize artificial genetic polymers hold great promise for advancing
future applications in synthetic biology. However, engineering natural polymerases to …
future applications in synthetic biology. However, engineering natural polymerases to …
Nucleic acids and their analogues for biomedical applications
F Wang, P Li, HC Chu, PK Lo - Biosensors, 2022 - mdpi.com
Nucleic acids are emerging as powerful and functional biomaterials due to their molecular
recognition ability, programmability, and ease of synthesis and chemical modification …
recognition ability, programmability, and ease of synthesis and chemical modification …
Ribozyme-catalysed RNA synthesis using triplet building blocks
RNA-catalyzed RNA replication is widely believed to have supported a primordial biology.
However, RNA catalysis is dependent upon RNA folding, and this yields structures that can …
However, RNA catalysis is dependent upon RNA folding, and this yields structures that can …