Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …

The steadfast advance of the synthetic biology field has enabled scientists to use genetically
engineered cells, instead of small molecules or biologics, as the basis for the development …

Synthetic gene circuits that precisely control human cell function could expand the
capabilities of gene-and cell-based therapies. However, platforms for developing circuits in …

Allosteric regulation of protein function is widespread in biology, but is challenging for de
novo protein design as it requires the explicit design of multiple states with comparable free …

Receptors enable cells to detect, process and respond to information about their
environments. Over the past two decades, synthetic biologists have repurposed physical …

Efforts to construct synthetic networks in living cells have been hindered by the limited
number of regulatory components that provide wide dynamic range and low crosstalk. Here …

Gene regulation is a complex and tightly controlled process that defines cell identity, health
and disease, and response to pharmacologic and environmental signals. Recently …

The ability to artificially control transcription is essential both to the study of gene function
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …

The design of modular protein logic for regulating protein function at the posttranscriptional
level is a challenge for synthetic biology. Here, we describe the design of two-input AND …

There is a growing need to enhance our capabilities in medical and environmental
diagnostics. Synthetic biologists have begun to focus their biomolecular engineering …