The power of any genetic model organism is derived, in part, from the ease with which gene
expression can be manipulated. The short generation time and invariant developmental …

C. elegans is used extensively as a model system in the neurosciences due to its well
defined nervous system. However, the seeming simplicity of this nervous system in …

Experimental manipulation of protein abundance in living cells or organisms is an essential
strategy for investigation of biological regulatory mechanisms. Whereas powerful techniques …

Neurons are highly polarized cells that face the fundamental challenge of
compartmentalizing a vast and diverse repertoire of proteins in order to function properly …

In principle, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9
allows genetic tags to be inserted at any locus. However, throughput is limited by the …

The capability to conditionally inactivate gene function is essential for understanding the
molecular basis of development. In gene and mRNA targeting approaches, protein products …

We developed infrared laser–evoked gene operator (IR-LEGO), a microscope system
optimized for heating cells without photochemical damage. Infrared irradiation causes …

As developmental biologists in the age of genome editing, we now have access to an ever-
increasing array of tools to manipulate endogenous gene expression. The auxin-inducible …

Transgenes are prone to progressive silencing due to their structure, copy number, and
genomic location. In C. elegans, repressive mechanisms are particularly strong in the …

The auxin-inducible degradation system in C. elegans allows for spatial and temporal
control of protein degradation via heterologous expression of a single Arabidopsis thaliana …