[HTML][HTML] Understanding the invisible hands of sample preparation for cryo-EM
Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …
A review of microfluidic-based mixing methods
Z Li, B Zhang, D Dang, X Yang, W Yang… - Sensors and Actuators A …, 2022 - Elsevier
Microfluidic-based mixing methods have aroused increasing attention due to their
tremendous potential in bio-related and materials science fields. Achieving mixing based on …
tremendous potential in bio-related and materials science fields. Achieving mixing based on …
Reducing effects of particle adsorption to the air–water interface in cryo-EM
Most protein particles prepared in vitreous ice for single-particle cryo-electron microscopy
(cryo-EM) are adsorbed to air–water or substrate–water interfaces, which can cause the …
(cryo-EM) are adsorbed to air–water or substrate–water interfaces, which can cause the …
[HTML][HTML] Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification
RBG Ravelli, FJT Nijpels, RJM Henderikx… - Nature …, 2020 - nature.com
The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current
sample preparation protocols, such as sample waste and lack of reproducibility. Here, we …
sample preparation protocols, such as sample waste and lack of reproducibility. Here, we …
Applications of acoustofluidics in bioanalytical chemistry
Acoustics has a broad spectrum of applications, ranging from noise cancelation to ultrasonic
imaging. In the past decade, there has been increasing interest in developing acoustic …
imaging. In the past decade, there has been increasing interest in developing acoustic …
Better, faster, cheaper: recent advances in cryo–electron microscopy
Cryo–electron microscopy (cryo-EM) continues its remarkable growth as a method for
visualizing biological objects, which has been driven by advances across the entire pipeline …
visualizing biological objects, which has been driven by advances across the entire pipeline …
Integrated ratiometric fluorescence probe-based acoustofluidic platform for visual detection of anthrax biomarker
J Wu, P Chen, J Chen, X Ye, S Cao, C Sun, Y Jin… - Biosensors and …, 2022 - Elsevier
The sensitive detection of dipicolinic acid (DPA) as an excellent biomarker of Bacillus
anthracis, especially through visual point-of-care testing, is significant for accurate and rapid …
anthracis, especially through visual point-of-care testing, is significant for accurate and rapid …
Current limitations to high-resolution structure determination by single-particle cryoEM
E D'Imprima, W Kühlbrandt - Quarterly reviews of biophysics, 2021 - cambridge.org
CryoEM has become the method of choice for determining the structure of large
macromolecular complexes in multiple conformations, at resolutions where unambiguous …
macromolecular complexes in multiple conformations, at resolutions where unambiguous …
[PDF][PDF] Shake-it-off: a simple ultrasonic cryo-EM specimen-preparation device
Although microscopes and image-analysis software for electron cryomicroscopy (cryo-EM)
have improved dramatically in recent years, specimen-preparation methods have lagged …
have improved dramatically in recent years, specimen-preparation methods have lagged …
[HTML][HTML] Automated vitrification of cryo-EM samples with controllable sample thickness using suction and real-time optical inspection
RI Koning, H Vader, M van Nugteren… - Nature …, 2022 - nature.com
The speed and efficiency of data collection and image processing in cryo-electron
microscopy have increased over the last decade. However, cryo specimen preparation …
microscopy have increased over the last decade. However, cryo specimen preparation …