Global signatures of protein and mRNA expression levels

R de Sousa Abreu, LO Penalva, EM Marcotte… - Molecular …, 2009 - pubs.rsc.org
Cellular states are determined by differential expression of the cell's proteins. The
relationship between protein and mRNA expression levels informs about the combined …

Integrating multiple 'omics' analysis for microbial biology: application and methodologies

W Zhang, F Li, L Nie - Microbiology, 2010 - microbiologyresearch.org
Recent advances in various 'omics' technologies enable quantitative monitoring of the
abundance of various biological molecules in a high-throughput manner, and thus allow …

Exponentially modified protein abundance index (emPAI) for estimation of absolute protein amount in proteomics by the number of sequenced peptides per protein* s

Y Ishihama, Y Oda, T Tabata, T Sato, T Nagasu… - Molecular & Cellular …, 2005 - ASBMB
To estimate absolute protein contents in complex mixtures, we previously defined a protein
abundance index (PAI) as the number of observed peptides divided by the number of …

GMD@ CSB. DB: the Golm metabolome database

J Kopka, N Schauer, S Krueger, C Birkemeyer… - …, 2005 - academic.oup.com
Metabolomics, in particular gas chromatography–mass spectrometry (GC–MS) based
metabolite profiling of biological extracts, is rapidly becoming one of the cornerstones of …

Absolute protein expression profiling estimates the relative contributions of transcriptional and translational regulation

P Lu, C Vogel, R Wang, X Yao, EM Marcotte - Nature biotechnology, 2007 - nature.com
We report a method for large-scale absolute protein expression measurements (APEX) and
apply it to estimate the relative contributions of transcriptional-and translational-level gene …

Community proteomics of a natural microbial biofilm

RJ Ram, NC VerBerkmoes, MP Thelen, GW Tyson… - Science, 2005 - science.org
Using genomic and mass spectrometry–based proteomic methods, we evaluated gene
expression, identified key activities, and examined partitioning of metabolic functions in a …

Protein abundance profiling of the Escherichia coli cytosol

Y Ishihama, T Schmidt, J Rappsilber, M Mann, FU Hartl… - BMC genomics, 2008 - Springer
Background Knowledge about the abundance of molecular components is an important
prerequisite for building quantitative predictive models of cellular behavior. Proteins are …

Global proteomic profiling of native outer membrane vesicles derived from Escherichia coli

EY Lee, JY Bang, GW Park, DS Choi, JS Kang… - …, 2007 - Wiley Online Library
Gram‐negative bacteria constitutively secrete native outer membrane vesicles (OMVs) into
the extracellular milieu. Although recent progress in this area has revealed that OMVs are …

Binding of silver nanoparticles to bacterial proteins depends on surface modifications and inhibits enzymatic activity

NS Wigginton, A Titta, F Piccapietra… - … science & technology, 2010 - ACS Publications
Here we describe results from a proteomic study of protein− nanoparticle interactions to
further the understanding of the ecotoxicological impact of silver nanoparticles (AgNPs) in …

Proteomics in gram‐negative bacterial outer membrane vesicles

EY Lee, DS Choi, KP Kim… - Mass spectrometry reviews, 2008 - Wiley Online Library
Gram‐negative bacteria constitutively secrete outer membrane vesicles (OMVs) into the
extracellular milieu. Recent research in this area has revealed that OMVs may act as …